Effect of SHP-1 knockout in airway epithelial cells on emphysema phenotype in chronic obstructive pulmonary disease in mice

Objective To construct and characterize conditional Src homology region 2 protein tyrosine phosphatase 1(SHP-1)knockout mice in airway epithelial cells and to observe the effect of defective SHP-1 expression in airway epithelial cells on the emphysema phenotype in chronic obstructive pulmonary disease(COPD).Methods To detect the expression of SHP-1 in the airway epithelium of COPD patients,CRISPR/Cas9 technology was used to construct SHP-1flox/flox transgenic mice,which were mated with airway epithelial Clara protein 10-cyclase recombinase and estrogen receptor fusion transgenic mice(CC10-CreER^(+/+)),and after intraperitoneal injection of tamoxifen,airway epithelial SHP-1 knockout mice were obtained(SHP-1^(max/fox) CC10-CreER^(+/+),SHP-1^(Δ/Δ)).