D-阿洛酮糖固定化酶的制备及色谱分离系统的优化

Preparation of Allulose Immobilized Enzyme and Optimization of Chromatographic Separation System

D-阿洛酮糖是一种理想的低热量甜味剂,主要通过D-阿洛酮糖3-差向异构酶(D-Allulose3-Epimerase,DAEase)进行制备。为了实现大规模、高效的商业化生产,以Co^(2+)型树脂为固定化载体、壳聚糖溶液对DAEase进行交联,得到固定化酶;利用固定化酶和凝胶型聚苯乙烯钙型色谱树脂的色谱分离系统进行阿洛酮糖料的制备,确定影响转化率的关键因素并进行优化。本研究结果有助于提高D-阿洛酮糖的生产效率,并为未来D-阿洛酮糖的大规模生产提供潜在的固定化酶策略。

D-Allulose is an ideal low-calorie sweetener,mainly prepared by using D-allulose 3-epimerase(DAEase).In order to achieve large-scale and efficient commercial production,Co^(2+)resin was used as immobilized carrier and chitosan solution was cross-linked to DAEase to obtain immobilized enzyme.The chromatographic separation system of immobilized enzyme and gel-type polystyrene calcium chromatographic resin was used to prepare allulose.The key factors affecting the conversion rate were determined and optimized.The results of this study may contribute to the cost reduction of d-Allulose industrial production and provide a potential immobilized enzyme strategy for future large-scale manufacturing of d-Allulose.