ALDH8A1在胃癌组织中的表达及其临床意义

Expression and clinical significance of ALDH8A1in gastric cancer tissues

目的探究重组表达醛脱氢酶8A1(ALDH8A1)在胃癌组织中的表达情况及与临床病理参数的关系,并分析其对患者远期预后的影响。方法纳入2012年3月—2018年4月在蚌埠医科大学第一附属医院接受根治术治疗的109例胃癌患者,采用免疫组化法分析ALDH8A1在胃癌及癌旁组织的表达情况,并运用统计学方法分析其与临床病理特征的关系;采用Kaplan-Meier曲线分析ALDH8A1的表达对胃癌患者术后5年生存率的影响。结果ALDH8A1在胃癌组织中的表达明显高于癌旁组织(P<0.001)。CEA≥5μg/L、CA19-9≥37 kU/L、癌细胞类型为腺癌、T3~4期及N2~3期患者ALDH8A1表达水平更高(P<0.05)。Kaplan-Meier曲线分析及多因素分析显示ALDH8A1高表达(HR=2.674,95%CI:1.293~5.533,P=0.008)可能会增加胃癌患者术后的死亡率。ROC曲线表明以2.555为截点值,ALDH8A1对胃癌患者预后具有较高的预判价值(灵敏度为68.97%,特异度为86.27%)。GO和KEGG富集分析显示ALDH8A1与细胞减数分裂、DNA结合和细胞周期等信号通路有关。结论ALDH 8 A 1在胃癌组织中高表达并促进胃癌的恶性进展,与预后不良相关。

Objective To investigate the expression of aldehyde dehydrogenase 8 family member A1(ALDH8A1)in gastric cancer tissues and its relationship with clinicopathological parameters,and to analyze its effect on the long-term prognosis of patients.Methods A total of 109 patients with gastric cancer who underwent radical resection in the First Affiliated Hospital of Bengbu Medical University from March 2012 to April 2018 were enrolled.Immunohistochemistry was used to analyze the expression of ALDH8A1in gastric cancer tissues and adjacent tissues,and statistical methods were used to analyze its relationship with clinicopathological features.Kaplan-Meier Plotter curve was used to analyze the effect of ALDH8A1expression on the 5-year survival rate of patients with gastric cancer after surgery.Results The expression of ALDH8A1in gastric cancer tissues was significantly higher than that in adjacent tissues(P<0.001).The high expression rate of ALDH8A1was higher in patients with CEA≥5μg/L,CA19-9≥37 kU/L,adenocarcinoma,T3-4 stage and N2-3 stage(P<0.05).Kaplan-Meier Plotter curve analysis and multivariate analysis showed that high ALDH8A1expression(HR=2.674,95%CI:1.293-5.533,P=0.008)may increase the mortality of patients with gastric cancer after surgery.ROC curve showed that with 2.555 as the cut-off value,ALDH8A1had a high predictive value for the prognosis of gastric cancer patients(sensitivity was 68.97%,specificity was 86.27%).GO and KEGG enrichment analysis showed that ALDH8A1was related to cell meiosis,DNA binding and cell cycle signaling pathways.Conclusion ALDH8A1is highly expressed in gastric cancer tissues and promotes the malignant progression of gastric cancer and is associated with poor prognosis.