4种细胞因子在桥本甲状腺炎和甲状腺乳头状癌中的表达及相关性研究

Expression and correlation of four kinds of cytokines in patients with Hashimoto's thyroiditis or papillary thyroid carcinoma

目的分析血清白细胞介素-2(IL-2)、白细胞介素-12p70(IL-12P70)、白细胞介素-10(IL-10)、白细胞介素-17(IL-17)在桥本甲状腺炎(HT)和甲状腺乳头状癌(PTC)中的表达差异,探讨4种细胞因子在HT和PTC中的作用。方法选取2022年7月—2023年6月浙江省立同德医院收治的HT患者30例(HT组),PTC患者35例(PTC组),健康体检者30例(对照组)。采用流式免疫荧光发光法检测各组血清IL-2、IL-12P70、IL-10、IL-17的水平。结果与对照组比较,PTC组患者血清IL-2、IL-12P70、IL-17显著升高(P<0.05),HT组血清IL-2、IL-12P70、IL-17升高,IL-10降低,差异有统计学意义(P<0.05)。与HT组比较,PTC组血清IL-10显著升高[(9.46±2.22)pg/mL vs.(7.44±1.97)pg/mL,P<0.05]。HT组甲状腺过氧化物酶抗体(TPOAb)与IL-10呈负相关关系,与IL-17呈正相关关系(P<0.05),甲状腺球蛋白抗体(TgAb)与IL-10呈负相关关系(P<0.05)。采用甲状腺全切除与采用患侧腺体合并峡部切除的PTC患者术后血清IL-2、IL-12P70、IL-10、IL-17比较差异均无统计学意义(P>0.05)。结论PTC患者和HT患者血清IL-2、IL-12P70、IL-17高表达,同时HT患者血清IL-10低表达,并与TPOAb和TgAb存在一定的相关性。采用甲状腺全切除和患侧腺体合并峡部切除的PTC患者术后4种细胞因子水平无显著变化。

Objective To analyzed the expression of serum Interleukin-2(IL-2),Interleukin 12 P70(IL-12P70),interleukin-10(IL-10)and interleukin-17(IL-17)in Hashimoto's thyroiditis carcinoma(HT)and papillary thyroid carcinoma(PTC),to investigate the role of 4 kinds of cytokines in HT and PTC.Methods Thirty patients with Hashimoto's thyroiditis,35 patients with papillary thyroid carcinoma,and thirty healthy individuals were selected from Tongde Hospital of Zhejiang Province from July 2022 to June 2023.They were divided into HT group,PTC group,and healthy control group.The serum levels of IL-2,IL-12P70,IL-10,and IL-17 were detected by flow cytometry.Results Compared with the healthy control group,the serum levels of IL-2,IL-12P70,and IL-17 were significantly higher in both the PTC and HT groups(P<0.05).Compared with the HT group,the serum level of IL-10 was significantly increased in the PTC group[(9.46±2.22)pg/mL vs.(7.44±1.97)pg/mL,P<0.05].In the HT group,thyroid peroxidase antibody(TPOAb)levels were negatively correlated with the level of IL-10 and positively correlated with the level of IL-17(P<0.05).The level of thyroglobulin antibody(TgAb)was negatively correlated with the level of IL-10(P<0.05).The levels of IL-2,IL-12P70,IL-10,and IL-17 in thyroid tumor patients showed no significant differences after total thyroidectomy or thyroid gland with isthmus resection(P>0.05).Conclusion The serum levels of IL-2,IL-12P70,and IL-17 elevated in PTC and HT patients,while the serum levels of IL-10 decreased in HT patients and correlated with TPOAb and TgAb levels.There were no significant changes in the levels of these cytokines after total thyroidectomy or thyroid gland with isthmus resection in patients with PTC.