右美托咪定对小鼠脑出血后氧化应激脑损伤及NOX4表达的影响

Effects of dexmedetomidine on oxidative stress brain injury and NOX4 expression after mouse intracerebral hemorrhage

目的探讨右美托咪定对小鼠脑出血后氧化应激脑损伤及NADPH氧化酶(NOX)4表达的影响。方法小鼠经腹腔注射右美托咪定30 min后制备脑出血模型,检测小鼠神经运动功能、脑含水量、NOX4 mRNA和NOX4蛋白表达水平及氧化应激水平。结果与对照组比较,脑出血组小鼠神经运动功能评分提升(P<0.05),脑水含量增加(P<0.05),NOX4 mRNA和NOX4蛋白表达水平明显增加(P<0.05),氧化应激水平升高;而右美托咪定组小鼠神经运动功能评分明显下降(P<0.05),脑含水量明显减少(P<0.05),NOX4 mRNA和NOX4蛋白表达水平明显减少(P<0.05),氧化应激水平降低。结论右美托咪定可以抑制脑出血后NOX4表达,减轻小鼠氧化应激脑损伤。

Objective To explore the effects of dexmedetomidine on oxidative stress brain injury and nicotinamide adenine dinucleotide phosphate oxidase(NOX)4 expression after intracerebral hemorrhage in mouse.Methods The mouse intracerebral hemorrhage model was prepared after 30 min intraperitoneal injection of dexmedetomidine.The neurological motor function,brain water content,NOX4 mRNA content,NOX4 protein expression level and oxidative stress levels were detected.Results Compared with the control group,the neurological motor function score in the intracerebral hemorrhage group was significantly increased(P<0.05),the brain water content was increased(P<0.05),the NOX4 mRNA and NOX4 protein expression levels were significantly increased(P<0.05),the oxidative stress level was increased;but the neurological motor function score in the dexmedetomidine group was significantly decreased(P<0.05),the brain water content was significantly decreased,the NOX4 mRNA and NOX4 protein expression levels were significantly decreased(P<0.05)and the oxidative stress level was decreased.Conclusion Dexmedetomidine could inhibit the NOX4 expression after intracerebral hemorrhage and alleviate the oxidative stress brain injury in mice.