虎耳草对小鼠B16细胞酪氨酸酶活性的影响

The Effects of Tyrosinase Activity of Mouse Melanoma Cell B16 on Saxifraga Extracts

为了阐明虎耳草不同极性溶剂提取物对小鼠B16黑色素瘤细胞增殖及酪氨酸酶活性的影响,用虎耳草提取物干预B16细胞,利用倒置显微镜观察给药后细胞的形态和数量,通过噻唑蓝(MTT)法分析虎耳草对细胞增殖活性的影响,通过多巴氧化法分析虎耳草对细胞内酪氨酸酶活性的影响。研究结果表明,虎耳草不同溶剂提取物对B16细胞的增殖活性均表现出一定的抑制作用,呈现浓度依赖性,虎耳草样品1~4对细胞增殖活性产生50%的抑制(IC_(50))时对应的质量浓度分别为74.52,103.48,109.60和65.54μg/mL,样品4抑制细胞增殖能力最强。虎耳草样品1~5对B16细胞内酪氨酸酶活性均表现为抑制作用,其中样品1~2和4~5的IC_(50)值依次为163.79,107.36,362.64以及307.50μg/mL,样品2抑制细胞内酪氨酸酶能力最好。综上所述,虎耳草不同极性溶剂提取物对小鼠B16黑色素瘤细胞增殖和细胞内酪氨酸酶活性都存在一定的抑制作用。

The extracts of Saxifraga with different polar solvents were prepared,and their effects on the proliferation and tyrosinase activity of mouse B16 melanoma cells were studied in this paper.The samples were used to intervene mouse melanoma cell B16.The morphology and quantity of mouse B16 melanoma cells were observed by inverted microscope.The cell viability was detected by thiazolyl blue(MTT)method,and tyrosinase activity in B16 melanoma cells were determined by dopa oxidation method.The results showed that different solvent extracts of Saxifraga could inhibit the proliferation of B16 cells in a concentration dependent manner.Compared with the control group,the corresponding concentrations of Saxifraga samples 1-4 were 74.52,103.48,109.60 and 65.54μg/mL,respectively,when half inhibition(IC_(50))of cell proliferation activity was produced.And sample 4 showed the strongest inhibition of cell proliferation.All Saxifraga samples showed inhibitory effect on tyrosinase activity in B16 melanoma cells,and the IC_(50) values of samples 1-2 and 4-5 were 163.79,107.36,362.64 and 307.50μg/mL,respectively,and sample 2 had the best inhibition ability on tyrosinase activity in cells.Based on the above research,we believe that the different polar solvent extracts of Saxifraga can inhibit the proliferation and tyrosinase activity of mouse B16 melanoma cells.