优化古树秋茶干茶中粗蛋白的提取方法与实际应用

Optimization of Extraction Method for Crude Protein in Large Leaf Autumn Tea and Its Practicality

探究临沧古树秋茶中粗蛋白质的高效提取方法,并研究地理因素对粗蛋白提取率的影响,以提高茶叶资源的价值化利用。采用碱溶酸沉法进行蛋白质提取,优化提取工艺,探究提取条件的影响,从商业角度出发,以获取最佳结果。研究表明,提取pH对提取结果有显著影响,而提取时间的变化对结果影响较小。在优化的提取条件:提取料液比1︰30 g/mL、提取温度50℃、提取时间90 min、提取pH 7.5下,蛋白质提取率达44.24%。通过优化的提取工艺可提高粗蛋白质的提取率,实现能源、成本和生产时间的显著节约,为古树秋茶的高值化利用提供依据,有望为茶叶产业的可持续发展作出贡献。

Explore the efficient extraction method of crude protein from Lincang ancient tree autumn tea,and study the influence of geographical factors on the extraction rate of crude protein,so as to improve the value utilization of tea resources.The protein extraction was performed by the basic acid sedimentation method,and the extraction process was optimized,with the focus on the influence of extraction conditions,and from a commercial perspective to obtain the best results.The studies showed that pH extraction had a significant effect on the extraction results,while the change in the extraction time had less effect on the results.Under the optimized extraction condition of extraction solution ratio 1∶30 g/mL,extraction temperature 50℃,extraction time 90 min,and extraction pH 7.5,the excellent results were obtained,and the protein extraction rate was as high as 44.24%.By optimizing the extraction process,the extraction rate of crude protein is improved,and the significant savings of energy,cost and production time are realized,which provides a basis for the high value utilization of ancient tree autumn tea,and is expected to contribute to the sustainable development of the tea industry.