黄芪、川芎不同比例配伍对黄酮类成分提取动力学的影响

Effects of Different Proportions of Astragali Radix and Chuanxiong Rhizoma on the Extraction Kinetics of Flavonoids

目的研究黄芪和川芎不同比例配伍对黄酮类成分的提取动力学过程的影响。方法建立黄酮成分(以芦丁为对照品)的含量测定方法,分别动态测定2 h内黄芪与川芎不同比例配伍(1∶1、1∶2、1∶3、1∶4、1∶5、2∶1、3∶1、4∶1、5∶1)的提取液中黄酮类成分的浓度,并根据菲克第一扩散定律,分别建立提取动力学模型,计算其提取速率常数,比较其溶出动力学差异性。结果黄芪与川芎不同比例配伍对黄酮类成分的提取速率和浓度均有显著影响,黄酮类成分的提取动力学模型均符合一级动力学方程特征,其中黄芪∶川芎为3∶1时提取速率最快,川芎∶黄芪2∶1时提取速率最慢,川芎∶黄芪为3∶1时黄酮类成分的平衡浓度最高,黄芪∶川芎比例为5∶1时最低。结论黄芪与川芎的不同比例配伍对黄酮类成分的提取速率和浓度有显著影响。

OBJECTIVE To study the effect of different proportion compatibility of Astragali Radix and Chuanxiong Rhizoma on the extraction kinetics of flavonoids.METHODS The content determination method of flavonoids(with rutin as the control substance)was established,and the concentrations of flavonoids in the extracts of Astragali Radix membranicum and Chuanxiong Rhizoma in different proportions(1∶1,1∶2,1∶3,1∶4,1∶5,2∶1,3∶1,4∶1,5∶1)were determined dynamically within 2 h,respectively.The extraction kinetics model was established according to Feck's first diffusion law.The extraction rate constant was calculated and the difference of dissolution kinetics was compared.RESULTS The compatibility of different proportions of Astragali Radix and Chuanxiong Rhizoma had significant effects on the extraction rate and concentration of flavonoids.The extraction kinetics models of flavonoids were consistent with the characteristics of the first-order kinetic equation.The extraction rate was the fastest when Astragali Radix∶Chuanxiong Rhizoma was 3∶1,the extraction rate was the slowest when Chuanxiong Rhizoma∶Astragali Radix was 2∶1.The equilibrium concentration of flavonoids was the highest when Chuanxiong Rhizoma∶Astragali Radix was 3∶1,when the ratio of Astragali Radix∶Chuanxiong Rhizoma was 5∶1,it was the lowest.CONCLUSION The compatibility of different proportions of Astragali Radix and Chuanxiong Rhizoma has a significant effect on the extraction rate and concentration of flavonoids.