淫羊藿苷通过激活内质网应激抑制胃癌细胞的增殖、侵袭和迁移

Icariin inhibits proliferation,invasion and migration of gastric cancer cells by activating endoplasmic reticulum stress

目的研究淫羊藿苷(icariin,ICA)对胃癌(gastric cancer,GC)恶性进展的影响。方法采用细胞计数试剂盒-8(cell countingkit-8,CCK-8)、集落形成、划痕闭合、Transwell试验和流式细胞术分别评估胃癌细胞AGS的存活率、增殖、迁移、侵袭和凋亡。然后,采用蛋白质印迹(Western blotting,WB)检测ICA作用下AGS细胞中凋亡相关蛋白和内质网(endoplasmic reticulum,ER)应激相关蛋白的表达水平。随后,在暴露于ICA和内质网应激抑制剂4-苯丁酸(4-phenylbutyric acid,4-PBA)的AGS细胞中再次进行上述功能检测,以确认ICA对ER应激的影响。结果ICA显著降低AGS细胞的活力、增殖、迁移和侵袭能力,同时诱导AGS细胞凋亡。此外,ICA处理提高了AGS细胞内质网应激的标记物。然而,4-PBA逆转了ICA对AGS细胞活力、增殖、迁移、侵袭和凋亡的影响,并伴有ER应激标记物的表达降低。结论ICA通过激活ER应激诱导GC细胞凋亡,抑制GC细胞的增殖、迁移和侵袭。

Objective To research the effect of icariin(ICA)on the malignant progression of gastric cancer(GC).Methods The survival rate,proliferation,migration,invasion and apoptosis of AGS cells were evaluated by cell counting kit-8(CCK-8),colony formation,scratch closure,Transwell test and flow cytometry,respectively.Then,the expression levels of apoptosis-related proteins and endoplasmic reticulum(ER)stress-related proteins in AGS cells treated with ICA were detected by Western blotting(WB).Subse-quently,the above functional tests were performed again in AGS cells exposed to ICA and ER stress inhibitor 4-phenylbutyric acid(4-PBA)to confirm the effect of ICA on ER stress.Results ICA significantly reduced the viability,proliferation,migration and invasion of AGS cells and induced apoptosis of AGS cells.In addition,ICA treatment increased the markers of ER stress in AGS cells.However,4-PBA reversed the effects of ICA on the viability,proliferation,migration,invasion and apoptosis of AGS cells,accompanied by de-creased expression of ER stress markers.Conclusion ICA induced GC cell apoptosis by activating ER stress and inhibits GC cell prolif-eration,migration and invasion.