3种具有D-A-π-A构型化合物的合成及细胞成像研究

Synthesis and Cellular Imaging Studies of Three Compounds with D-A-π-A Configuration

分别以三苯胺、咔唑、香豆素为构筑单元,设计合成了3种具有D-A-π-A构型的化合物A1、A2、A3,并通过质谱、红外、核磁共振氢谱和碳谱对3种化合物的结构进行了表征.结合理论计算,利用紫外可见吸收光谱和荧光发射光谱系统研究了化合物 A1、A2 和 A3 的光学性能.结果表明3种化合物的光学性质良好.MTT实验和激光共聚焦成像实验表明,3种化合物细胞毒性低,可用于细胞成像.

In this study,three compounds A1,A2 and A3 with D-A-π-A configuration were synthesized using triphenylamine,carbazole and coumarin as building blocks,respectively.The structures of the three compounds were characterized by mass spectrometry,infrared spectroscopy,hydrogen nuclear magnetic resonance spectroscopy and carbon spectroscopy.Combined with theoretical calculations,the optical properties of compounds A1,A2 and A3 were investigated using UV-visible absorptionand fluorescence emission spectroscopy systems.The results showed that the three compounds have favorable optical characteristics.MTT assay and laser confocal imaging indicated that the three compounds had low cytotoxicity and could be used for cellular imaging.