肌酸相关基因在小鼠中枢神经系统中的表达模式分析

Analysis of the Expression Patterns of Creatine-related Genes in the Mouse Central Nervous System

肌酸能够维持细胞中ATP的平衡,在细胞能量代谢方面发挥了关键作用.肌酸合成和转运异常会引起肌酸缺乏综合征,造成神经发育迟缓和神经退行性疾病.截至目前,肌酸相关调控基因在中枢神经系统中的表达模式尚不清楚.前期通过筛选转录组数据库,初步发现肌酸相关基因Slc6a8、Gatm和Gamt主要表达在少突胶质谱系细胞中.为验证这一发现,通过RNA原位杂交,分别检测了Slc6a8、Gatm、Gamt在不同发育阶段小鼠脑和脊髓组织中的表达模式.结合免疫组化,进一步确认Slc6a8、Gatm、Gamt主要表达于分化后的少突胶质细胞中.上述研究结果表明,在中枢神经系统中,少突胶质细胞可能是主要的肌酸合成细胞,对整个中枢神经系统的肌酸稳态维持及能量代谢具有重要的支持功能.

Creatine is able to maintain adenosine triphosphate(ATP)balance in cells and plays a key role in cellular energy metabolism.Abnormal synthesis and transport of creatine can lead to creatine deficiency syndrome(CDS),resulting in neurodevelopmental delay and neurodegenerative diseases.To date,the expression patterns of creatine-related genes in the central nervous system(CNS)remain poorly identified.By screening the transcriptome database,it was initially found that creatine related genes Slc6a8,Gatm and Gamt were predominantly expressed in oligodendrocyte(OL)lineage cells.To test this finding,the expression patterns of Slc6a8,Gatm and Gamt in mouse brain and spinal cord tissues at different developmental stages were examined through RNA in situ hybridization.Immunohistochemistry confirmed that Slc6a8,Gatm and Gamt were mainly expressed in differentiated oligodendrocytes.The results suggest that oligodendrocytes may be the major creatine synthesizing cells in the CNS.Oligodendrocytes play an important role in the maintenance of creatine homeostasis and energy metabolism in the whole central nervous system.