两种肿瘤化疗药物对七种常见致病菌的抑菌性分析

Analysis of the antimicrobial activity of two kind of cancer chemotherapy drugs against seven common pathogenic bacteria

目的研究细菌与肿瘤化疗药物共培养下的生长情况及奥沙利铂与盐酸吉西他滨这两种肿瘤化疗药物对7种常见致病菌的抑菌作用。方法采用大肠埃希菌(ATCC25922)与一系列浓度的奥沙利铂共培养,在一定的时间间隔(0、2、4、6、8、10、12、24 h)监测细菌,绘制生长曲线;采用96孔板的微量细菌药敏试验方法,7种常见致病菌的标准菌株大肠埃希菌(ATCC25922)、肺炎克雷伯菌(ATCC700623)、金黄色葡萄球菌(ATCC25913)、铜绿假单胞菌(ATCC27853)、产气肠杆菌(ATCC13048)、鲍曼不动杆菌(ATCC19606)、粪肠球菌(ATCC29212)分别与两种肿瘤化疗药物奥沙利铂、盐酸吉西他滨进行药敏试验,检测其MIC50和MIC90。结果在高浓度的奥沙利铂(800 mg/L和1000 mg/L)存在下,细菌的生长明显受到抑制,出现负增长;中间浓度的奥沙利铂(80 mg/L)存在下,细菌的生长曲线明显较对照组有所减缓;低浓度的奥沙利铂(0.032 mg/L、1.6 mg/L)存在下的生长曲线与对照几乎吻合。奥沙利铂对大肠埃希菌、肺炎克雷伯菌、金黄色葡萄球菌、鲍曼不动杆菌、产气肠杆菌、铜绿假单胞菌、粪肠球菌的MIC50分别是:125、31.25、125、125、125、31.25、31.25 mg/L;MIC90则分别为250、125、1000、500、250、250、125 mg/L。盐酸吉西他滨对大肠埃希菌、肺炎克雷伯菌、金黄色葡萄球菌、鲍曼不动杆菌、产气肠杆菌、铜绿假单胞菌、粪肠球菌的MIC50分别是:1000、1000、62.5、500、500、62.5、62.5 mg/L;MIC90则分别为2000、4000、1000、1000、2000、1000、500 mg/L。结论奥沙利铂在一定浓度上对大肠埃希菌的生长存在抑制作用;奥沙利铂和盐酸吉西他滨在体外试验中,对7种常见致病菌大肠埃希菌、肺炎克雷伯菌、金黄色葡萄球菌、鲍曼不动杆菌、产气肠杆菌、铜绿假单胞菌、粪肠球菌均具有不同程度的抑菌作用。

Objective To research the growth of bacteria co-cultured with tumor chemotherapy drugs and to research the antimicrobial activity of the two cancer chemotherapy drugs against the seven common patho-genic species.Methods Escherichia coli(ATCC25922)was co-cultured with a series of oxaliplatin concentra-tions,and the bacteria were monitored at a certain time interval(0,2,4,6,8,10,12,24 h)to draw the growth curve.The drug sensitivity of seven common pathogenic bacteria(Escherichia coli,Klebsiella pneumoniae,Staphylococcus aureus,Acinetobacter baumannii,Enterobacter aerogenes,Pseudomonas aeruginosa,Entero-coccus faecalis)were analyzed with two cancer chemotherapy drugs(Oxaliplatin and Gemcitabine HCl)using 96-well TC plates;The MIC50 and MIC90 of the two drugs against these bacteria were tested.Results In the presence of high concentrations of oxaliplatin(800mg/L and 1000mg/L),the growth of bacteria was signifi-cantly inhibited and showed negative growth.In the presence of oxaliplatin at intermediate concentration(80mg/L),the growth curve of bacteria slowed down significantly compared with the control group.The growth curve of oxaliplatin with low concentration(0.032mg/L,1.6mg/L)was almost consistent with the control.The MIC50 of Oxaliplatin against E.coli,Klebsiella pneumoniae,S.aureus,Acinetobacter baumannii,E.aerogenes,P.aeruginosa,Ent.faecalis were 125,31.25,125,125,125,31.25,31.25 mg/L,respectively;MIC90 were 50,125,1000,500,250,250,125 mg/L,respectively.The MIC50 of Gemcitabine HCl against E.coli,Klebsiella pneumoniae,S.aureus,Acinetobacter baumannii,E.aerogenes,P.aeruginosa,Ent.faecalis were 1000,1000,62.5,500,500,62.5,62.5 mg/L,respectively;MIC90 were 2000,4000,1000,1000,2000,1000,500 mg/L,respectively.Conclusion Oxaliplatin can inhibit the growth of Escherichia coli at certain concen-tration;In vitro,Oxaliplatin and Gemcitabine HCl can inhibit the grown of the seven common pathogenic bac-teria in different degrees.