藤梨根多糖对小鼠酒精性肝损伤的保护作用

Protective Effects of Polysaccharide from Actinidia Chinensis Planch on Alcoholic Liver Injury in Mice

目的:探究藤梨根(Actinidia chinensis root)多糖提取物对慢性酒精性肝损伤(Alcoholic liver injury,ALI)模型小鼠的保护作用研究。方法:采用超声法提取藤梨根多糖,随机将60只雄性健康昆明小鼠分为空白对照组、模型组、阳性对照组、藤梨根多糖低、中、高剂量组,除空白组外,其余各组小鼠连续灌胃56°红星二锅头酒15天,复制急性酒精性肝损伤模型后处死,检测各组小鼠血清中谷丙转氨酶(alanine aminotransferase,ALT)、谷草转氨酶(aspartate aminotransferase,AST)、甘油三酯(triglyceride,TG)、总胆固醇(total cholesterol,TC)含量及肝组织中丙二醛(malondialdehyde,MDA)、谷胱甘肽过氧化物酶(glutathioneperoxidase,GSH-Px)、超氧化物歧化酶(superoxide dismutase,SOD)水平,以及血清中肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)和白细胞介素-6(interleukin-6,IL-6)含量,计算小鼠肝脏指数,并对肝组织进行病理切片观察。结果:实验结果表明,与模型组相比,藤梨根多糖各浓度组血清AST、ALT、TG、TC水平显著降低(P<0.05);肝脏SOD和GSH水平上升显著(P<0.05);IL-6、TNF-α和MDA含量下降,有统计学意义(P<0.05);肝脏病理学改变明显改善。结论:藤梨根多糖能缓解快速大量饮酒引起的肝损伤,其机制可能与其抗氧化作用相关。

Objective:Exploring the protective effect of polysaccharide from Actinidia Chinensis Planch on alcoholic liver injury(ALI)model mice.Method:Ultrasound was used to extract polysaccharides from Actinidia chinensis root.Sixty healthy male Kunming mice were randomly divided into six groups:blank control group,model group,positive control group,low,medium and high dose groups of polysaccharide from Actinidia Chinensis Planch.Except for the blank group,all other groups of mice were continuously gavaged with 56°Red Star Erguotou wine for 15 days.The acute alcoholic liver injury model was replicated and killed.The serum levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST),triglyceride(TG),total cholesterol(TC),superoxide dismutase(SOD),Glutathione peroxidase(GSH-Px),Malondialdehyde(MDA)level and tumor necrosis factor-α(TNF-α)and Interleukin-6(IL-6)content in liver tissue were measured.Calculate the mouse liver index,and observe pathological sections of liver tissue.Results:The experimental results showed that compared with the model group,the levels of serum AST,ALT,TG,and TC in the different concentration groups of polysaccharides from Actinidia chinensis root were significantly reduced(P<0.05);The levels of SOD and GSH in the liver increased significantly(P<0.05);IL-6,TNF-αand MDA concentration decreased(P<0.05);The pathological changes of the liver were significantly improved.Conclusion:polysaccharide from Actinidia Chinensis Planch can alleviate liver damage caused by rapid and excessive alcohol consumption,and their mechanism may be related to their antioxidant effects.