四种不同品种番茄果实不同组织的抗氧化活性研究

Antioxidant Activity of Different Tissues of Four Varieties of Tomato Fruit

为探究番茄果实中不同组织抗氧化活性物质的含量、活性及二者的相关性,高效利用番茄果实中的抗氧化活性物质,系统比较了2种大果番茄(‘丰收128’‘DRK0568’)、1种小果番茄(‘千禧’)、1种实验室模式番茄(‘AC’)不同组织(果皮、果肉和种子)中抗氧化活性物质的含量与抗氧化活性之间的关系。结果表明,果皮是番茄红素含量最多的组织,多酚类及类黄酮类物质在果皮和种子中含量较高,抗坏血酸在果皮和果肉中的含量较高。4个番茄品种中,‘千禧’小番茄总酚、总黄酮、抗坏血酸等抗氧化活性物质的含量均高于其他3个品种。果皮组织中亲水提取物的2,2-联氨-双-3-乙基苯并噻唑啉-6-磺酸(2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid),ABTS)阳离子自由基清除率与总黄酮含量显著相关,铁离子还原抗氧化活性与总酚含量、抗坏血酸含量均极显著相关,亲脂提取物抗氧化活性与总酚和总黄酮和番茄红素含量均极显著相关;种子中的亲水提取物和亲脂提取物抗氧化活性与总黄酮和番茄红素含量有较高的相关性;果肉中的亲水提取物抗氧化活性与抗坏血酸含量的相关性较高,亲脂提取物抗氧化活性与番茄红素含量的相关性最高。综上可知,番茄的果皮和种子含有较丰富的抗氧化活性成分,抗氧化能力相对较高。本研究结果可为高效利用番茄中的抗氧化活性物质及健康饮食提供指导。

In order to explore the content,activity and correlation of antioxidant active substances in different tissues in tomato fruits,and utilize the antioxidants efficiently,this study systematically compared the relationship between the content of antioxidants and antioxidant activity in different tissues(peel,pulp and seed)of four varieties of tomato fruits,including two large fruit tomatoes(‘Fengshou 128’‘DRK0568’),one small fruit tomato(‘Qianxi’)and one laboratory model tomato(‘AC’).The results showed that the peel was the tissue that had the highest lycopene content.Polyphenols and flavonoids were higher in peel and seeds.Ascorbic acid was found in higher amounts in peel and flesh.Among the four tomato varieties,the contents of total phenols,total flavonoids and ascorbic acid in‘Qianxi’variety were significantly higher than those of the other three varieties.The 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid(ABTS)),cation free radical scavenging rate of the hydrophilic extract in peel tissue was significantly correlated with the content of total flavonoids,while the ferric reducing ability was significantly correlated with the content of both total phenols and ascorbic acid.The antioxidant activity of lipophilic extracts was significantly correlated with the contents of total phenols,total flavonoids and lycopene.The antioxidant activity of hydrophilic and lipophilic extracts in seed showed a high correlation with the contents of total phenols and total flavonoids.The antioxidant activity of the hydrophilic extracts in flesh was highly correlated with the ascorbic acid content,while the antioxidant activity of lipophilic extracts showed the highest correlation with the lycopene content.In conclusion,the peel and seeds of tomato contained rich antioxidant active components and showed relatively high antioxidant capacity.This study can provide guidance for the efficient utilization of antioxidant active substances in tomato and a healthy diet.