幽门螺杆菌相关性胃炎关键基因的筛选及潜在治疗中药预测

Screening of key genes for Helicobacter pylori-associated gastritis and prediction of potential traditional Chinese medicines for treating it

基于生物信息学筛选幽门螺杆菌(Helicobacter pylori,Hp)相关性胃炎的关键基因、分析通路机制、预测潜在治疗中药。首先从GEO数据库获取GSE60427与GSE60662数据集,借助R软件筛选出差异表达基因并进行富集分析,通过STRING数据库和Cytoscape软件进一步筛选出关键基因,利用UALCAN数据库、GEPIA数据库对关键基因进行表达验证,在Coremine Medical数据库预测筛选潜在中药。最后筛选出344个差异表达基因,与白细胞粘附、T细胞活化、免疫反应、趋化因子受体结合等生物功能有关,通过Th17细胞分化、Th1和Th2细胞分化等信号通路。得到的10个关键基因是PTPRC、TNF、ITGB2、FCGR3A、CD19、LCK、LCP2、CD48、CTLA4、IL7R。结合中医“毒邪”理论,将预测的潜在中药分为健脾解毒、化湿解毒、活血解毒、清热解毒四大类,可为治疗Hp相关性胃炎,抑制“炎-癌”转化,防治胃癌提供思路。

This study aims to screen key genes associated with gastritis associated with Helicobacter pylori(Hp),analyze the pathway mechanism,and predict potential traditional Chinese medicines for treating it based on bioinformatics.The GSE60427 and GSE60662 datasets were obtained from the GEO database,the differentially expressed genes were screened out with R software and enriched and analyzed,the key genes were further screened out by STRING database and Cytoscape software,and the expression verification analysis of key genes were performed by UALCAN database,GEPIA database,and traditional Chinese medicines were predicted and screened in Coremine Medical database.A total of 344 differentially expressed genes were screened for biological functions such as leukocyte adhesion,T cell activation,immune response,chemokine receptor binding,and other signaling pathways through Th17 cell differentiation,Th1 and Th2 cell differentiation.The ten key genes obtained are PTPRC,TNF,ITGB2,FCGR3A,CD19,LCK,LCP2,CD48,CTLA4,IL7R.Combined with the theory of"toxicity"in Chinese medicine,the predicted traditional Chinese medicine is divided into four groups:strengthening the spleen and detoxification,resolving dampness and detoxification,invigorating the blood and detoxification and clearing away heat and detoxification,and these medicines can provide ideas for the treatment of Hp-related gastritis,inhibition of"inflammation-cancer"transformation,and prevention and treatment of gastric cancer.