类枯草杆菌蛋白酶基因GmSBT1在大豆共生固氮中的功能

Function of subtilases GmSBT1 in symbiotic nitrogen fixation of soybean

为研究SBT(subtilases)家族蛋白在豆科植物共生固氮中的作用机制,分别利用生物信息学分析、时空表达定位、GUS染色定位和基因沉默等方法来研究GmSBT1在大豆-根瘤菌共生中的作用。结果显示:GmSBT1受根瘤菌特异性诱导,在成熟根瘤中高量表达,且在根瘤皮层以及含菌细胞中发挥功能。GmSBT1-RNAi植株地上鲜质量、瘤质量、固氮酶活显著降低。结果表明,GmSBT1蛋白对根瘤的形成和发育以及根瘤的固氮具有重要的作用。

Bioinformatics analysis,spatiotemporal expression localization,GUS staining localization and gene silencing were used to study the mechanism of SBT family proteins in the soybean-rhizobia symbiosis to investigate the function of the GmSBT1 gene in symbiotic nitrogen fixation between rhizobia and host plants.The results showed that the GmSBT1 gene was specifically induced by rhizobia and only expressed at high levels in the nodules,possibly functioned in the rhizobia cortex and bacterial cells.RNA interference significantly reduced the fresh weight,nodule weight,and nitrogenase activity aboveground.It is indicated that the GmSBT1 protein plays an important role in the formation and development of nodules,and the nitrogen fixation in nodules as well.