响应面法优化大曲发酵力测定条件的研究

Optimization of Determination Conditions of Daqu Fermentation Power by Response Surface Method

以糖化液为发酵底物,发酵力为评价指标,在糖化液底物、糖度、pH值、发酵时间和发酵温度5个单因素试验结果的基础上,采用Box-Behnken中心组合设计试验,对大曲发酵条件进行优化。结果表明,以高粱糖化液为发酵底物时,更适宜大曲微生物生长,当无氧发酵时间为96 h时,发酵力趋于稳定;响应面回归模型及统计分析得出大曲发酵最佳条件:糖化液pH5、糖化液浓度7°Bx、发酵温度30℃,在此条件下大曲发酵力最大。研究结果可为大曲发酵力的检测提供理论参考。

Taking the saccharification liquid as the fermentation substrate and the fermentation power as the evaluation index,on the basis of single-factor tests on different saccharification liquid substrate,sugar content of the saccharification liquid,pH value of the saccharification liquid,fermentation time and fermentation temperature,the Box-Behnken center combination design experiments were carried out to optimize the fermentation conditions of Daqu.The results showed that the sorghum saccharification liquid was more suitable for the growth of Daqu microorganisms;when the anaerobic fermentation time was 96 hours,the fermentation power tended to be stable.Response surface regression model and statistical analysis showed that the optimal conditions for Daqu fermentation were as follows:the pH value of the saccharification liquid was 5,the concentration of the saccharification liquid was 7°Bx,and the fermentation temperature was 30℃.Under these conditions,the fermentation power of the Daqu was the highest.The research results has provided theoretical reference for the detection of Daqu fermentation power.