荆条的化学成分研究

The chemical constituents of Vitex negundo var.heterophylla

目的:研究荆条(Vitex.negundo var.heterophylla)的化学成分。方法:采用溶剂萃取法,硅胶、ODS、Sephadex LH-20以及半制备型HPLC等多种柱色谱分离方法进行分离纯化,通过理化常数测定、波谱数据分析鉴定化合物的结构。结果:从荆条叶、种子的95%乙醇提取物中分离得到5个化合物,分别鉴定为5,4'-二羟基-3,6,7-三甲氧基黄酮、5,4'-二羟基-3,6,7,8-四甲氧基黄酮、对羟基苯甲醛、对羟基苯甲酸、对羟基苯甲酸甲酯。结论:通过对荆条的化学成分进行研究,丰富了荆条的化学组成及其可能发挥药效的物质基础。

Objective:To investigate the chemical constituents of Vitex negundo var.heterophylla.Methods:The chemical constituents were separated and purified by silica gel,opening ODS column chromatography,Sephadex LH-20,and semi-preparative HPLC.Their structures were elucidated on the basis of physico-chemical properties and spectroscopic data.Results:Five compounds were isolated from the 95%ethanol extract of the leaves and seeds of Vitex negundo var.heterophylla.They were identified as 5,4'-dihydroxy-3,6,7-trimethoxyflavone,5,4'-dihydroxy-3,6,7,8-tetramethoxyflavone,p-hydroxybenzaldehyde,p-hydroxybenzoic acid,and p-hydroxybenzoic acid methyl ester.Conclusion:The study of the chemical constituents of Vitex negundo var.heterophylla enriches the chemical composition of Vitex negundo var.heterophylla and the material basis for its potential pharmacological effects.