OBI感染中单独抗-HBc+和非单独抗-HBc+献血者S区变异情况分析

Occult HBV among anti-HBc +alone and not alone samples:mutation analysis of S Gene

目的 了解本市献血人群隐匿性乙肝(OBI)感染中单独抗-HBc+和非单独抗-HBc+献血者S区变异情况。方法 采用ELISA与NAT筛查OBI标本,扩增和外送测序,获得单独抗-HBc+20份及非单独抗-HBc+25份测序标本。结果 本市献血者OBI检出率为0.10%(155/161 045),其中,抗-HBc阳性率为74.19%(115/155);OBI检出率与性别无关,随献血者年龄增高而增高(P<0.05);单独抗-HBc阳性率为22.58%(35/155),非单独抗-HBc阳性率为51.61%(80/155);45份OBI测序标本中,B基因型占比为73.33%(33/45),C型为20.00%(9/45),单独抗-HBc+组献血者突变位点多于非单独抗-HBc+组,位于MHR上的S114T和V168A变异率有差异(P<0.05)。结论 衢州市OBI感染基因型以B型为主,单独抗-HBc+组献血者突变位点高于非单独抗-HBc+组,可能更适合作为血液OBI筛查指标之一。

Objective To investigate the status of occult hepatitis B virus infection(OBI) among blood donors in Quzhou,Zhejiang,and to analyze the mutation of S region in blood donors with anti-HBc+ alone and non anti-HBc+ alone.Methods The OBI samples were screened by ELISA and NAT;the HBV DNA was amplified and sequenced;20 anti-HBc+ alone and 25 not anti-HBc+ alone samples were obtained.Results The detection rate of OBI in Quzhou was 0.10%(155/161 045),and the positive rate of anti-HBc was 74.19%(115/155).The detection rate of OBI increased with the age of blood donors(P<0.05),but was not related to gender.The positive rate of anti-HBc+ alone was 22.58%(35/155),and that of not anti-HBc alone was 51.61%(80/155).Among the 45 OBI sequencing samples,the proportion of B and C genotype was 73.33%(33/45) and 20.00%(9/45),respectively.The mutation sites of blood donors in the anti-HBc+ alone group were more than those in the not anti-HBc+ alone group,and the mutation rates of S114T and V168A on MHR were significantly different(P<0.05).Conclusion The genotype of OBI infection in Quzhou is mainly type B.The mutation sites of blood donors with anti-HBc+ alone are higher than those with not anti-HBc+ alone,which may be more suitable as one of the OBI screening indicators.