龈沟液炎性因子及TSP-1在拔牙正畸患者中的表达情况及发生牙周疾病的影响因素分析

Analysis of the Expression of Inflammatory Factors and TSP-1 in Gingival Crevicular Fluid in Orthodontic Patients Undergoing Tooth Extraction and the Influencing Factors of Periodontal Disease Occurrence

目的:探讨龈沟液炎性因子及TSP-1在拔牙正畸患者中的表达情况及发生牙周疾病的影响因素。方法:选取我院2020年8月到2023年8月收治的80例拔牙正畸治疗患者进行回顾性分析,分别取所有患者正畸前、正畸后1个月、3个月及正畸结束时的龈沟液样本检测肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、血小板反应蛋白-1(TSP-1)表达水平。随后依照患者正畸治疗过程中是否患有牙周疾病将其分为牙周疾病组(n=36)及非牙周疾病组(n=44),对比两组患者正畸前及正畸结束时的TNF-α、IL-1β、IL-6、TSP-1表达水平,对比两组患者一般情况,采用logistics回归模型分析拔牙正畸患者牙周疾病的影响因素。结果:80例拔牙正畸患者中正畸后1个月、3个月TNF-α、IL-1β、IL-6、TSP-1水平升高,正畸后3个月到正畸结束时TNF-α、IL-1β、IL-6、TSP-1水平趋于平稳,但正畸后1个月、3个月及结束时明显高于正畸前(P<0.05);牙周疾病组与非牙周疾病组患者正畸前、正畸后TNF-α、IL-1β、IL-6、TSP-1表达水平对比差异显著,牙周疾病组明显高于非牙周疾病组(P<0.05);牙周疾病组及非牙周疾病组患者性别、年龄、BMI对比无明显差异(P>0.05),牙周疾病组及非牙周疾病组患者正畸治疗时间、拔牙数量、正畸方式、口腔清洁度对比差异显著(P<0.05);logistic回归分析结果表明:TNF-α、IL-1β、IL-6、TSP-1、正畸方式、口腔清洁度为拔牙正畸患者牙周疾病的独立危险因素(P<0.05)。结论:拔牙正畸患者随着正畸时间延长龈沟液炎性因子及TSP-1水平明显升高,且TNF-α、IL-1β、IL-6、TSP-1、正畸方式、口腔清洁度为拔牙正畸患者牙周疾病的独立危险因素。

Objective:To explore the expression of inflammatory factors and TSP-1 in gingival crevicular fluid in patients undergoing orthodontic extraction,and the influencing factors of periodontal disease.Methods:Eighty patients admitted to our hospital from August 2020 to August 2023 for orthodontic treatment of extracted teeth were retrospectively analyzed,and gingival sulcus fluid samples were taken from all the patients before orthodontic treatment,1 month after orthodontic treatment,3 months after orthodontic treatment,and at the end of orthodontic treatment to detect the tumor necrosis factor-alpha(TNF-alpha),interleukin-1beta(IL-1beta),interleukin-6(IL-6),and platelet reactive protein-1(TSP-1)expression levels.Subsequently,the patients were divided into periodontal disease group(n=36)and non-periodontal disease group(n=44)according to whether they suffered from periodontal disease during orthodontic treatment,compared the expression levels of TNF-α,IL-1β,IL-6,and TSP-1 of the patients in the two groups before orthodontic treatment and at the end of orthodontic treatment,and compared the general conditions of the patients in the two groups,and then analyzed the influence factors of periodontal diseases in patients with extracted teeth in orthodontics with the use of logistic regression model.Disease Influencing Factors.Results:TNF-α,IL-1β,IL-6,and TSP-1 levels were elevated at 1 month and 3 months after orthodontic treatment in 80 patients with extracted teeth,and TNF-α,IL-1β,IL-6,and TSP-1 levels leveled off from 3 months to the end of orthodontic treatment,but were significantly higher than those before orthodontic treatment at 1 month,3 months,and at the end of orthodontic treatment(P<0.05);there were significant differences in the expression levels of TNF-α,IL-1β,IL-6,and TSP-1 in patients in the periodontal diseases group compared to those in the non periodontal diseases group,and the expression levels in the periodontal diseases group were significantly higher than those in the non periodontal diseases group(P<0.05).There were significant differences in the expression levels of TNF-α,IL-1β, IL-6, and TSP-1 between patients in the pre-orthodontic and post-orthodontic groups, and the periodontal disease group was significantlyhigher than the non-periodontal disease group (P<0.05);there were no significant differences in the comparison of gender, age,and BMI between patients in the periodontal disease group and the non-periodontal disease group (P>0.05), and there were no significantdifferences in the duration of orthodontic treatment, number of extractions, oral cavity, oral cavity, and oral health of patients in the periodontaldisease group and the non-periodontal disease group (P<0.05). number, orthodontic method, and oral cleanliness were comparedsignificantly (P<0.05);logistic regression analysis showed that TNF-α, IL-1β, IL-6, TSP-1, orthodontic method, and oral cleanliness wereindependent risk factors for periodontal disease in patients with extracted teeth and orthodontic treatment (P<0.05). Conclusion: Inflammatoryfactors of gingival crevicular fluid and TSP-1 levels increased significantly in orthodontic patients with prolonged orthodontictime, and TNF-α, IL-1 β, IL-6, TSP-1, orthodontic mode and oral cleanliness were independent risk factors for periodontal disease in orthodonticpatients.