基于网络药理学探讨甘西鼠尾草药理作用及作用机制

Pharmacological mechanism exploration of Salviae prʐewalskii Maxim based on network pharmacology

目的 基于网络药理学分析甘西鼠尾草的药理作用及作用机制。方法 采用国内外数据库结合中药系统药理学数据库与分析平台(TCMSP)筛选甘西鼠尾草的活性成分,采用Swiss Target Prediction、Pharm Mapper数据库筛选活性成分的靶点,利用比较毒理基因组学(CTD)数据库收集靶点的相关信息,采用Cytoscape 3.8.2软件构建活性成分-靶点、靶点-疾病网络,通过STRING 11.5数据库构建靶点的蛋白质-蛋白质相互作用(PPI)网络,最后使用DAVID、Bioconductor数据库对靶点进行基因本体(GO)和京都基因与基因组百科全书(KEGG)分析,并构建靶点-通路网络。结果 共筛选出甘西鼠尾草活性成分26个,活性成分对应靶点218个;GO功能富集结果233个,主要涉及药物结合、三磷酸腺苷(ATP)结合、类固醇结合等;KEGG通路富集结果161条,主要涉及癌症、心血管系统、神经系统、内分泌系统等信号通路。结论 本研究从整体水平阐述了甘西鼠尾草的药理作用及其作用机制,为进一步深入研究甘西鼠尾草药理作用的分子机制提供了依据和基础。

Objective To analyze the pharmacological action and mechanism of Salviae przewalskii Maxim based on network pharmacology.Methods The active components of Salviae przewalskii Maxim were screened by using domestic and foreign databases combined with Traditional Chinese Medicine System Pharmacology Database and Analysis Platform(TCMSP),and the targets of active components were screened by using Swiss Target Prediction and Pharm Mapper databases.Comparative Toxicology Genomics(CTD)database was used to collect the relevant information of the target,and Cytoscape 3.8.2 software was used to construct the active ingredient-target,targetdisease network,and protein-protein interaction(PPI)network of the target was constructed through STRING 11.5 database.Finally,DAVID and Bioconductor databases were used to analyze the target gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG),and the target-pathway network was constructed.Results A total of 26 active components were selected from Salviae przewalskii Maxim,and 218 targets were identified.There were 233 GO functional enrichment results,which mainly involved drug binding,adenosine triphosphate(ATP)binding and steroid binding.It showed 161 KEGG pathway enrichment results,mainly involved in cancer,cardiovascular system,nervous system,endocrine system and other signaling pathways.Conclusion This study described the pharmacological action and mechanism of Salviae przewalskii Maxim from the whole and provided the basis and basis for further research on the molecular mechanism of pharmacological action of the medical plant.