乳腺癌组织miR-1247-5p表达及其靶基因生物信息学预测研究

Expression of miR-1247-5p in breast cancer tissue and bioinformatics prediction of its target gene

目的:探讨微小核糖核酸-1247-5p(miR-1247-5p)在乳腺癌组织中的表达,并对其预测的靶基因进行生物信息学分析。方法:收集2019年6月至2020年12月本院手术治疗的104例乳腺癌患者癌组织及其癌旁组织。RT-PCR检测标本中miR-1247-5p的相对表达量;比较不同临床病理特征患者癌组织miR-1247-5p表达;预测miR-1247-5p的靶基因;采用DAVID数据库对miR-1247-5p靶基因进行功能和通路富集分析;String数据库对miR-1247-5p靶基因进行互作分析。结果:miR-1247-5p在乳腺癌组织中的表达水平较癌旁组织下调(P<0.05)。miR-1247-5p潜在靶基因38个,其靶基因功能主要富集于RNA聚合酶Ⅱ启动子的转录调控、基因表达/转录调控、质膜部分、突触小体、蛋白质结合、poly(A)RNA结合等;参与的通路主要富集于癌症通路、细胞因子与细胞因子受体的相互作用、磷脂酰肌醇3激酶(PI3K)-蛋白激酶B(Akt)信号通路等。磷酸酯酶与张力蛋白同源物(PTEN)、上皮生长因子受体(EGFR)、假尿苷酸合酶1(PUS1)的编码蛋白质在miR-1247-5p基因靶基因蛋白质互作网络(PPI)中关键节点位置。结论:miR-1247-5p在乳腺癌组织中的表达下调,其靶基因富集于多个生物学过程及与肿瘤相关的信号转导通路上,miR-1247-5p可能通过调控其靶基因的表达参与乳腺癌的发生发展过程。

Objective:To investigate the expression of miR-1247-5p(miR-1247-5p)in breast cancer tissues,and to analyze its predicted target genes by bioinformatics.Methods:The cancerous tissues and adjacent tissues were collected from 104 patients with breast cancer who received surgical treatment in our unit from June 2019 to December 2020.The relative expression of miR-1247-5p was detected by RT-PCR.The expression of miR-1247-5p in cancer tissues of patients with different clinicopathological characteristics was compared.The target genes of miR-1247-5p were predicted.The DAVID database was used for function and pathway enrichment of miR-1247-5p target genes.The String database was utilized to analyze the interactions of miR-1247-5p target genes.Results:The expression level of miR-1247-5p in breast cancer tissue was lower than that in adjacent tissue(P<0.05).There were 38 potential target genes of miR-1247-5p,and the functions of its target genes are mainly concentrated in transcriptional regulation of RNA polymeraseⅡpromoter,gene expression/transcriptional regulation,plasma membrane portion,synaptosome,protein binding,and poly(A)RNA binding,etc.The involved pathways were mainly enriched in cancer pathway,interaction between cytokines and cytokine receptors,and Phosphatidylinositol 3 kinase(PI3K)-protein kinase B(Akt)signal pathway,etc.Phosphatase and tensin homology(PTEN),epithelial growth factor receptor(EGFR),pseudouridine synthase 1(PUS1)encoding protein were found in the key node positions of miR-1247-5p gene target gene protein-protein interaction networks(PPI).Conclusion:miR-1247-5p is down-regulated in breast can-cer tissues,and its target genes are enriched in multiple biological processes and tumor-related signal transduction pathways.miR-1247-5p may participate in the occurrence and development of breast cancer by regulating the expression of its target genes.