基于离子色谱法快速测定血清中钠、钾、钙、镁4种阳离子水平

Rapid quantitative determination of content for four cations:sodium,potassium,calcium and magnesium in serum based on ion chromatography

目的建立并优化一种基于微波消解结合离子色谱法同时测定血清中钠、钾、钙、镁4种阳离子的方法,并对其性能进行评价。方法采用微波消解技术对血清样品进行前处理,使用离子色谱定量检测4种阳离子。参照美国临床和实验室标准协会(CLSI)发布的EP6-A和EP15-A3文件对建立的方法进行线性、精密度、加标回收率和正确度等性能验证。结果钠、钾、钙、镁4种离子的检测不精密度以变异系数(CV)表示,分别为0.10%~0.36%、0.15%~0.48%、0.22%~0.87%、0.21%~0.73%。4种阳离子的加标回收率均在97.3%~103.0%之间。参加RELA2020和RELA2021计划的分析结果偏差分别为-0.57%~-0.14%、-0.84%~-0.16%、-1.82%~-0.37%、-0.60%~0.34%,比对均通过。4种阳离子的检出限/定量限分别为0.0175/0.0583mmol/L、0.0005/0.0017mmol/L、0.0099/0.0329mmol/L、0.0011/0.0035mmol/L,表明经前处理后,其他杂质对测定无明显干扰。结论成功建立并优化了一种基于微波消解结合离子色谱法同时测定血清中钠、钾、钙、镁4种阳离子的方法。该方法精密度和正确度良好,操作简单,分析时间短,成本低,可用于临床血清电解质检测的质量改进。

Objective To establish and optimize a method for simultaneous determination for the contents of sodium,potassium,calci-um and magnesium ions in serum using microwave digestion combined with ion chromatography,and evaluate its performance.Methods Serum samples were pretreated by microwave digestion,and four cations were quantitatively determined using ion chromatography.The linearity,precision,recovery and accuracy of the established method were verified with reference to the EP6-A and EP15-A3 docu-ments issued by the Clinical and Laboratory Standards Institute(CLSI).Results The imprecisions of determinations for sodium,po-tassium,calcium and magnesium ions expressed as coefficient of variation(CV)were 0.10%to 0.36%,0.15%to 0.48%,0.22%to 0.87%and 0.21%to 0.73%,respectively.The recoveries with adding standard of the four cations were between 97.3%and 103.0%.Referred to RELA2020 and RELA2021,the deviations of the analysis results were-0.57%to-0.14%,-0.84%to-0.16%,-1.82%to-0.37%,and-0.60%to 0.34%for sodium,potassium,calcium,and magnesium,respectively,and all the comparisons were pas-sed.The limits of detection/quantification were 0.0175/0.0583 mmol/L,0.0005/0.0017 mmol/L,0.0099/0.0329 mmol/L and 0.0011/0.0035 mmol/L for the four cations,indicating that after pretreatment,no significantly interfere from other impurities for the results of determination was found.Conclusion A method for simultaneous determination of sodium,potassium,calcium and magnesi-um ions in serum was successfully established and optimized using microwave digestion combined with ion chromatography.The method showed good precision and accuracy,simple operation,short time consumption and low cost,thus it could be used for quality improve-ment of clinical electrolyte testing in serum.