基于标准汤剂的鹅不食草HPLC特征图谱相关性研究

Correlation analysis on HPLC characteristic spectrum of Centipeda minima based on standard decoction

目的建立基于标准汤剂的鹅不食草HPLC特征图谱检测方法, 对其药材、饮片、标准汤剂、中间体、配方颗粒的相关性进行评价, 为鹅不食草配方颗粒质量标准的制定提供依据。方法采用HPLC法建立鹅不食草药材、饮片、标准汤剂、中间体、配方颗粒特征图谱, 采用中药指纹图谱相似度评价系统进行评价, 确定共有峰, 并通过聚类分析、主成分分析和正交偏最小二乘判别分析(OPLS-DA)确定药材、饮片、标准汤剂、中间体、配方颗粒的相关性, 筛选影响差异的标志性成分。结果鹅不食草药材、饮片、标准汤剂、中间体、配方颗粒特征图谱与各自的对照图谱相似度较高;药材、饮片、标准汤剂、中间体、配方颗粒中均呈现14个特征峰, 指认出11个特征峰;聚类分析和主成分分析结果一致, 标准汤剂与中间体、配方颗粒更为接近;OPLS-DA筛选得到6个VIP值大于1的差异性成分。结论该方法稳定、准确, 专属性强, 通过对药材、饮片、标准汤剂、中间体、配方颗粒特征图谱相关性的研究, 可为鹅不食草配方颗粒质量标准研究提供依据。

Objective To establish a HPLC characteristic spectrum method of Centipeda minima based on standard decoction;To evaluate the correlation of materia medica,decoction pieces,standard decoction,intermediates and formula granule;To provide a reference for the quality standard research of Centipeda minima formula granules.Methods The materia medica,decoction pieces,standard decoction,intermediates and formula granules of Centipeda minima were analyzed by a HPLC characteristic spectrum method,and the software"Similarity Evaluation System for Chromatographic Fingerprint of TCM"was employed to conform the common peaks.The clustering analysis(CA),principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA)were adopted to find out the correlation of materia medica,decoction pieces,standard decoction,intermediates and formula granules of Centipeda minima.The Iconic components which can make differences was screened at the same time.Results The characteristic chromatogram of materia medica,decoction pieces,standard decoction,intermediates and formula granules of Centipeda minima shared high similarity with the respective control chromatogram.Fourteen common peaks were confirmed,and eleven chromatographic peaks were identified.The results of CA and PCA were consistent,and the standard decoction,intermediates and formula granules of Centipeda minima were closer.Six differential components with VIP values greater than 1 were screened by OPLS-DA.Conclusions The method is stable,accurate and characteristic.The study can provide a reference for the quality standard research of Centipeda minima Formula Granules by analyzing the correlation of characteristic spectrum of materia medica,decoction pieces,standard decoction,intermediates and formula granules.