肥胖型2型糖尿病患者血清Glypican-4、Spexin及SFRP5表达水平及与胰岛素抵抗的关系

Expression of serum Glypican-4,Spexin and SFRP5 and their relationship with insulin resistance in obese type 2 diabetes patients

目的 探讨肥胖型2型糖尿病患者血清脂肪因子(Glypican-4)、人神经肽(Spexin)及分泌型卷曲蛋白5(SFRP5)表达水平,并分析其与胰岛素抵抗的关系。方法 选择2020年5月-2022年5月安徽省皖南康复医院接诊的45例肥胖型2型糖尿病患者为病例组,并选择同期于本院体检体重健康的2型糖尿病40例作为对照组,比较两组血清Glypican-4、Spexin、SFRP5、胰岛素相关指标[空腹胰岛素(FINS)、胰岛素抵抗指数(HOMA-IR)]水平,Spearman相关性分析Glypican-4、Spexin、SFRP5水平与FINS、HOMA-IR的相关性。结果 病例组血清Glypican-4、FINS、HOMA-IR水平[(1.04±0.41)ng/mL、(15.78±3.14) mU/L、(3.82±1.05)]显著高于对照组[(0.81±0.25) ng/mL、(11.30±3.51)mU/L、(2.42±1.12)],Spexin、SFRP5水平[(870.25±168.47) ng/L、(38.31±15.05)μg/L]显著低于对照组[(1553.08±287.98)ng/L、(48.89±13.58)μg/L],差异均有统计学意义(t=3.075、6.212、5.946、13.520、3.386,P均<0.05)。相关性分析结果显示,血清FINS、HOMA-IR与Glypican-4均成正相关(r=0.458、0.502,P均<0.05),与Spexin、SFRP5均成负相关(r=-0.385、-0.416,-0.408、-0.496,P均<0.05)。结论 肥胖型2型糖尿病患者血清Glypican-4、Spexin及SFRP5水平与胰岛素抵抗之间关系密切。

Objective To study the expression of serum adipokine(Glypican-4), human neuropeptide(Spexin) and secreted frizzin related protein 5(SFRP5) and their relationship with insulin resistance in obese type 2 diabetes patients.Methods A total of 45 obese patients with type 2 diabetes admitted to Wannan Rehabilitation Hospital of Anhui Province from May 2020 to May 2022 were selected as the case group, and 40 patients with type 2 diabetes with healthy body weight in the same hospital were selected as the control group. Compare the changes of serum Glypican-4, Spexin, SFRP5, insulin related indicators [fasting insulin(FINS), insulin resistance index(HOMA-IR)] and Spearman correlation was used to analyze the correlation between Glypican-4, Spexin, SFRP5 levels and FINS, HOM-IR. Results Serum Glypican-4, FINS and HOMAIR levels in case group [(1.04±0.41)ng/mL,(15.78±3.14)mU/L,(3.82±1.05)] were significantly higher than those in control group [(0.81±0.25)ng/mL,(11.30±3.51)mU/L,(2.42±1.12)];the levels of Spexin and SFRP5 [(870.25±168.47)ng/L,(38.31±15.05)μg/L] were significantly lower than those of control group [(1 553.08±287.98)ng/L,(48.89±13.58)μg/L];the differences were statistically significant(t=3.075, 6.212, 5.946, 13.520, 3.386;all P<0.05). Correlation analysis results showed that serum FINS and HOMA-IR were positively correlated with Glypican-4(r=0.458, 0.502;both P<0.05), and negatively correlated with Spexin and SFRP5(r=-0.385,-0.416;-0.408,-0.496;all P<0.05). Conclusion The expression of serum Glypican-4, Spexin and SFRP5 were closely related to insulin resistance in obese patients with type 2 diabetes.