蔬菜样品中有机磷农药的溶胶-凝胶固相纤维萃取条件优化研究

Optimization of Fabric Phase Sorptive Extraction Conditions for Organophosphorus Pesticides in Vegetable Samples

本文对溶胶-凝胶固相纤维萃取蔬菜中的有机磷农药的条件进行优化,结合气相色谱-质谱分析,用于快速测定豆类、番茄、茄子和卷心菜等蔬菜样品中的4种有机磷农药。在搅拌速度1200 r·min^(-1)、提取时间25 min、100μL乙酸乙酯解吸8 min、解吸2次的实验条件下,有机磷农药萃取效率最高。有机磷农药在番茄、卷心菜、茄子和豆类中的回收率分别为92.3%~98.9%、88.6%~97.9%、88.9%~95.6%和88.2%~97.3%,回收率值的分布范围很窄,表明优化后试验的灵敏度与重复性都很好。实际样品分析结果表明,该方法绿色、快速、经济可行,适合用于蔬菜样品中有机磷农药的测定。

In this paper,an experimental optimization of the fabric phase sorptive extraction method for the detection of organophosphorus pesticides in vegetables was carried out,combining gas chromatography and mass spectrometry for the rapid determination of four organophosphorus pesticides in vegetable samples,including beans,tomatoes,aubergines and cabbages.Under the experimental conditions of stirring speed 1200 r·min^(-1),extraction time 25 min,100μL ethyl acetate desorption for 8 min and desorption twice,the extraction efficiency of organophosphorus pesticides was the highest.The recoveries of the organophosphorus pesticides ranged from 92.3%to 98.9%in tomato,88.6%to 97.9%in cabbage,88.9%to 95.6%in aubergines,and 88.2%to 97.3%in beans,with a narrow distribution of the recovery values,which indicated that the optimized test had high sensitivity and reproducibility.The results of the actual sample analyses showed that the method is green,rapid,economically feasible and suitable for the determination of organophosphorus pesticides in vegetable samples.