异养小球藻ARTP诱变育种试验研究

Experimental Studies on Mutation of Heterotrophic Chlorella Using Atmospheric and Room Temperature Plasma Method

对异养小球藻进行常压室温等离子体(Atmospheric and Room Temperature Plasma,ARTP)诱变确定最佳诱变条件,在最佳诱变条件下筛选突变株,并开展突变株的摇瓶培养再验证试验、遗传稳定性试验、50 L发酵罐试验。结果表明,异养小球藻的最佳致死时间为50 s;得到6株突变株,且其细胞数多于出发菌株、质量浓度明显高于出发菌株;突变性能可以稳定遗传,突变株可提前24 h到达发酵终点,发酵液中油脂产量高于出发菌株。

The mutation of Heterotrophic Chlorella using atmospheric and room temperature plasma method was performed to determine the optimal mutagenesis conditions,the mutant strains were screened under the optimal mutagenesis conditions,and the mutant strains were reverified by shakingflask culture test,genetic stability test and 50 L fermenter test.The results showed that the optimal lethal time of Heterotrophic chlorella was 50 s.Six mutant strains were obtained,and their cell number and mass concentration were significantly higher than that of the original strain.The mutant strain could reach the end of fermentation 24 h in advance,and the oil yield in fermentation liquor was higher than that of the original strain.