摘要
目的 探究淋巴细胞活化基因-3(Lymphocyte activation gene-3,LAG3)分子对泡球蚴感染小鼠肝脏B淋巴细胞亚群及其功能的影响。方法 将野生型(C57-wild-type, WT)和LAG3基因敲除(LAG3-knockout, LAG3-KO)小鼠经肝门静脉注射泡球蚴原头节,感染12周免疫组织化学染色观察小鼠肝脏CD19和α-SMA表达及定位,流式细胞术检测小鼠肝脏B细胞及其各亚群和表面CD80、CD86、MHC-Ⅱ分子的表达情况。结果 泡球蚴感染12周小鼠肝脏LAG3-KO组CD19阳性区及面积占比略高于WT组,但差异均无统计学意义。α-SMA在LAG3-KO组阳性区及面积占比显著高于WT组(t=-3.224、-3.227,P均<0.05)。LAG3-KO组肝脏B细胞表达CD80和MHC-Ⅱ分子的比例显著上调,差异具有统计学意义(t=-2.379、-3.321,P均<0.05)。LAG3-KO组B2细胞占比显著高于WT组,差异有统计学意义(t=-2.695,P<0.05)。LAG3-KO组B1b细胞表达CD80比例亦显著上调,差异具有统计学意义(t=-5.315,P<0.001),而B2细胞表达CD80比例显著低于WT组,且差异具统计学意义(t=2.806,P<0.05)。LAG3-KO组B2细胞表达MHC-Ⅱ分子显著上调,且具统计学差异(t=-4.227,P<0.01)。结论 泡球蚴感染中期12周,LAG3分子影响小鼠肝脏B细胞亚群及功能,并以B2型淋巴细胞尤为显著,LAG3对B细胞的活化以及MHC-Ⅱ类分子表达产生抑制作用,提示其可能参与泡球蚴感染下的B细胞耗竭。
This study was aimed at investigating the effect of lymphocyte activation gene-3(LAG3)on liver B lymphocyte subsets and their functions in WT and LAG3-KO mice infected with Echinococcus multilocularis(E.multilocularis).In a mouse model of E.multilocularis infection,the expression and localization of CD19 andα-SMA in liver were detected by immunohistochemistry.CD80,CD86 and MHC-Ⅱmolecules expressed on B cells and their subsets in mice liver were detected by flow cytometry.After 12 weeks of infection,the area and percentage of CD19 in LAG3-KO group was slightly higher than that in WT group,but the difference was not statistically(t=-1.241、-1.237,P>0.05).The area and percentage ofα-SMA in LAG3-KO group was higher than that in WT group(t=-3.224、-3.227,P<0.05).The proportion of CD80 and MHC-Ⅱmolecules expressed on liver B cells in LAG3-KO group was up-regulated(t=-2.379,-3.321,P<0.05).The percentage of liver B2 cells in LAG3-KO group was higher than that in WT group(t=-2.695,P<0.05).The expression of CD80 on B1b cells in LAG3-KO group was significantly up-regulated(t=-5.315,P<0.001).The proportion of CD80 of B2 cells in LAG3-KO group was lower than that in WT group(t=2.806,P<0.05).The expression of MHC-Ⅱmolecule in B2 cells in LAG3-KO group was up-regulated(t=-4.227,P<0.01).It is suggested that LAG3 molecules affected the B cell subsets and function of mouse liver in the middle stage of E.multilocularis infection,especially B2 lymphocytes.LAG3 molecule exerted an inhibitory effect on the activation of B cells and the expression of MHC-class II molecules,suggesting that it may be involved in B cell exhaustion caused by E.multilocularis.
作者
郑旭然
邓冰清
康雪娇
李寅时
阿比旦·艾尼瓦尔
余倩
孜比姑·肉素
阿迪莱·多力坤
王茂林
王慧
张传山
李静
ZHENG Xu-ran;DENG Bing-qing;KANG Xue-jiao;LI Yin-shi;ABIDAN Ainiwaer;YU Qian;ZIBIGU Rousu;ADILAI Duolikun;WANG Mao-lin;WANG Hui;ZHANG Chuan-shan;LI Jing(School of Basic Medical Sciences,Xinjiang Medical University,Urumqi 830054,China;Institute of Clinical Medicine,The First Affiliated Hospital of Xinjiang Medical University,State Key Laboratory of Pathogenesis,Prevention and Treatment of High Incidence Diseases in Central Asia,Xinjiang Uygur Autonomous Region Key Laboratory of Echinococcosis,Urumqi 830054,China;Laboratory of High Incidence Disease Research in Xingjiang(Xinjiang Medical University),Ministry of Education,Urumqi 830054,China;Xinjiang Uygur Autonomous Region Key Laboratory of Molecular Biology for Endemic Diseases,Urumqi 830054,China)
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2024年第6期529-536,共8页
Chinese Journal of Zoonoses
基金
国家自然科学基金(No.82060370,No.82302561,No.82372279)
新疆维吾尔自治区自然科学基金(No.2022D01E51,No.2022D01D60)
新疆地区高发疾病研究教育部重点实验室开放课题资助项目(No.2023B03)。