HPLC法同时测定脑脉泰胶囊中12种成分的含量

Simultaneous content determination of twelve constituents in Naomaitai Capsules by HPLC

目的建立HPLC法同时测定脑脉泰胶囊中3′-羟基葛根素、2-羟基红花黄色素A、葛根素、葛根素芹菜糖苷、3′-甲氧基葛根素、大豆苷、二苯乙烯苷、木犀草苷、染料木苷、木犀草素、丹酚酸B、迷迭香酸的含量。方法分析采用KromasilC_(18)色谱柱(250mm×4.6mm,5μm);流动相乙腈-水(含1.0g/L磷酸),梯度洗脱;体积流量0.8mL/min;柱温30℃;检测波长280nm。再进行聚类分析、主成分分析。结果12种成分在各自范围内线性关系良好(r>0.9990),平均加样回收率97.07%~98.30%,RSD0.86%~1.82%。10批样品聚为4类,4种主成分累积方差贡献率达86.262%。结论该方法简便稳定,可靠准确,可为脑脉泰胶囊的质量控制提供科学依据。

AIM To establish an HPLC method for the simultaneous content determination of 3′⁃hydroxy⁃puerarin,hydroxysafflor yellow A,puerarin,puerarin apigenin,3′⁃methoxypuerarin,daidzein,stilbene glycoside,luteolin side,genistein side,luteolin B and rosmarinic acid in Naomaitai Capsules.METHODS The analysis was performed on a 30℃thermostatic Kromasil C_(18) column(250 mm×4.6 mm,5μm),with the mobile phase comprising of acetonitrile⁃water(containing 1.0 g/L phosphoric acid)flowing at 0.8 mL/min in a gradient elution manner,and the detection wavelength was set at 280 nm.Subsequently,principal component analysis and cluster analysis were made.RESULTS Twelve components showed good linear relationships within their own ranges(r>0.9990),whose average recoveries were 97.00%-99.00%with the RSDs of 0.86%-1.82%.Ten batches of samples were clustered into four categories,the accumulative variance contribution rate of four principal components reached 86.262%.CONCLUSION This simple,stable,reliable and accurate method can be used for the quality control of Naomaitai Capsules.