摘要
目的建立HPLC法同时测定知黄抗癌方中红景天苷、绿原酸、芒果苷、虎杖苷、盐酸小檗碱、异牡荆素、白藜芦醇的含量。方法分析采用SPOLARC_(18)色谱柱(4.6mm×250mm,5μm);流动相乙腈-0.1%磷酸,梯度洗脱;体积流量1.0mL/min;柱温30℃;检测波长275nm。结果7种成分在各自范围内线性关系良好(r≥0.9997),平均加样回收率91.32%~106.95%,RSD0.4%~2.9%。结论该方法简便快速,专属性好,可用于知黄抗癌方的质量控制。
作者
芮黎珺
杨喜梅
王瑶
刘梅
徐嘉
RUI Li-jun;YANG Xi-mei;WANG Yao;LIU Mei;XU Jia(Department of Pharmacy,Fudan University Shanghai Cancer Center Department of Oncology,Shanghai Medical College,Fudan University,Shanghai 200032,China;Shanghai Innovation Center of TCM Health Service,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Department of Assisted Reproduction,Shanghai Ninth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine,Shanghai 200011,China;Technology Transfer Center,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)
出处
《中成药》
CAS
CSCD
北大核心
2024年第7期2153-2156,共4页
Chinese Traditional Patent Medicine
基金
上海市科委生物医药科技支撑项目(18401931500)。
关键词
知黄抗癌方
化学成分
含量测定
HPLC
Zhihuang Kang’ai Prescription
chemical constituents
content determination
HPLC
