精神分裂症患者血清Klotho蛋白水平与炎症因子的相关性研究

Correlation between serum Klotho protein levels and inflammatory factors in patients with schizophrenia

目的:目的:观察精神分裂症患者血清Klotho蛋白水平及其与炎症因子的相关性。方法:纳入精神分裂症患者45例(观察组),健康对照45例(对照组),采用阳性与阴性症状量表(PANSS)评定患者精神症状严重程度,采用酶联免疫吸附技术(ELISA)测定所有受试者血清中Klotho蛋白和炎症因子IL-6、IL-10水平,使用全自动血细胞分析仪和生化分析仪测定其他炎症及抗炎指标。结果:观察组血清Klotho蛋白水平明显高于对照组(P<0.05);观察组炎症指标IL-6、WBC、CRP、MONO、NLR、MLR明显高于对照组(P<0.05),HNR明显低于对照组(P<0.05),但炎症指标IL-10、PLR在两组之间无显著差异;相关性分析显示精神分裂症患者血清Klotho蛋白水平与炎症因子IL-6和NLR成正相关(r=0.517,P<0.001;r=0.330,P<0.05)。结论:精神分裂症患者Klotho蛋白表达异常,且与炎症因子相关,Klotho蛋白可能参与精神分裂症的神经炎症反应过程。

Objective:To observe the serum Klotho protein level and its correlation with inflammatory factors in patients with schizophrenia.Methods:A total of 45 patients with schizophrenia(observation group)and 45 healthy controls(control group)were enrolled,the severity of mental symptoms was assessed by the positive and negative symptom scale(PANSS),the serum levels of Klotho protein and inflammatory factors IL-6 and IL-10 in all subjects were measured by enzyme-linked immunosorbent assay(ELISA),and other inflammatory and anti-inflammatory indexes were determined by automatic hematology analyzer and biochemical analyzer.Results:The serum level of Klotho protein in the observation group was significantly higher than that in the control group(P<0.05).The inflammatory indexes IL-6,WBC,CRP,MONO,NLR and MLR in the observation group were significantly higher than those in the control group(P<0.05),and the HNR was significantly lower than that in the control group(P<0.05),but there was no significant difference in the inflammatory indexes IL-10 and PLR between the two groups.Correlation analysis showed that serum Klotho protein levels were positively correlated with inflammatory cytokines IL-6 and NLR in patients with schizophrenia(r=0.517,P<0.001;r=0.330,P<0.05).Conclusion:The expression of Klotho protein in patients with schizophrenia is abnormal,and it is related to inflammatory factors,and Klotho protein may be involved in the neuroinflammatory response process of schizophrenia.