复合微生物菌剂对毛竹土壤细菌群落结构的影响

Soil Microbiome at Phyllostachys edulis Forest Affected by Application of Bioagent

【目的】探究复合微生物菌剂[产气肠杆菌(Enterobacter aerogenes)CT-B09-2、解淀粉芽孢杆菌(Bacillus amyloliquefaciens)JL-B06和乙酸钙不动杆菌(Acinetobacter calcoaceticus)WYS-A01-1]对毛竹土壤细菌群落结构和多样性的影响。【方法】以毛竹(Phyllostachys edulis)实生幼苗为研究对象,采用灌根的方式施用复合微生物菌剂,30 d后采集毛竹幼苗根际与非根际土壤样本,测定土壤理化性质,提取土壤总DNA并进行16S rRNA高通量测序,分析复合微生物菌剂对土壤细菌群落结构和多样性的影响。【结果】复合微生物菌剂可以有效提升土壤中速效磷的含量,调控土壤pH,提高根际土壤中物质代谢和碳化合物分解相关的功能活性。毛竹根际与非根际土壤共检测出26门、65纲、158目、253科、448属、674种微生物,主要优势菌门包括变形菌门Proteobacteria、拟杆菌门Firmicutes、放线菌门Actinobacteriota、酸杆菌门Acidobacteriota、绿弯菌门Chloroflexi等。施用复合微生物菌剂后,毛竹根际土壤微生物群落物种数目显著上升,非根际土壤样本无显著变化。【结论】复合微生物菌剂可以调节土壤矿质元素,改善土壤pH,调控细菌微生物群落组成。

【Objective】Effect of application of a microbial agent on the microbiome in soil at a Phyllostachys edulis forest was analyzed.【Method】In a P.edulis forest,a microbial solution of Enterobacter aerogenes CT-B09-2,Bacillus amyloliquefaciens JL-B06,and Acinetobacter calcoaceticus WYS-A01-1 was applied by root irrigation onto the soil around the seedlings.Rhizosphere and non-rhizosphere soil samples at the spots were collected 30 d after the application for physical and chemical analyses.Total DNA in soil was extracted,and 16S rRNA high-throughput sequencing performed to dissect the microbial community structure and diversity.【Results】The applied bioagent effectively increased the available phosphorus,regulated the pH,and improved the functional activities related to material metabolism and decomposition of carbon compounds in the rhizosphere soil.There were 26 phyla,65 classes,158 orders,253 families,448 genera,and 674 species detected in the soil samples with Proteobacteria,Firmicutes,Actinobacteriota,Acidobacteriota,and Chloroflexi being the dominant phyla.The application significantly increased the number of species in the microbial community only in the rhizosphere soil.【Conclusion】Application of the mixed microbial cultures significantly and positively affected the mineral contents,pH,and microbial community in the rhizosphere soil at the bamboo forest.