不同光质对香椿种子萌发、幼苗总黄酮合成及相关酶活性的影响

Effects of Different Lights on Seed Germination,Total Flavonoids Synthesis,and Related Enzyme Activities of Toona sinensis Seedlings

为探索不同光照对香椿幼苗生长、总黄酮合成及相关酶活性的影响,以‘红油椿’为试验材料,采用LED光源,对萌发种子及幼苗进行白光(CK)、蓝光(B)、红蓝复合光2:1(R2B1)、红蓝复合光1:1(R1B1)、红蓝复合光1:2(R1B2)、红光(R)处理,在不同光照处理15 d后对种子发芽指标、幼苗总黄酮合成以及相关酶活性进行了测定。结果表明,与对照组CK相比,红光为主的光质处理(R、R1B1、R2B1)促进了香椿种子萌发,其中R2B1处理全株干质量与鲜质量最高,与CK相比分别提高了55.06%、82.86%。与CK和R相比,其他光照处理均显著(P<0.05)提高了香椿幼苗中总黄酮的含量,其中B处理显著高于其他处理组,同时B处理显著(P<0.05)提高了苯丙氨酸解氨酶(PAL)、查尔酮合成酶(CHS)、查尔酮异构酶(CHI)的活性,与香椿叶片中总黄酮含量的变化呈显著正相关。因此,采用R2B1处理香椿种子能促进其萌发,采用B处理香椿幼苗能有效促进PAL、CHS、CHI酶活性以显著提高总黄酮的合成。

In order to explore the effects of different lights on seed germination,total flavonoids synthesis,and related enzyme activities of Toona sinensis seedlings,LED were applied to the seeds and seedlings of Toona sinensis.The experimental materials were‘Hongyou Toona sinensis’.White light(CK),blue light(B),red and blue light 2:1(R2B1),red and blue light 1:1(R1B1),red and blue light 1:2(R1B2),red light(R)were used for 15 days respectively,and the seed germination,total flavonoid synthesis and related enzyme activities in seedlings were measured.The results showed that compared to the control group(CK),the red light dominated illumination(R,R1B1,R2B1)promoted the seeds germination,and the dry and fresh weight of the whole plant treated with light(R2B1)increased by 55.06% and 82.86% respectively,which were the highest among all the treatments.Compared with CK and R,other light treatments significantly increased the total flavonoid content in Toona sinensis seedlings(P<0.05).Treatment B was significantly higher than other groups,and the activities of phenylalanine ammonia lyase(PAL),chalcone synthase(CHS),and chalcone isomerase(CHI)were also significantly increased(P<0.05),which showed a significant positive correlation with the changes in total flavonoid.Therefore,the germination of Toona sinensis seeds can be facilitated by the treatment of R2B1,and a significant enhancement in total flavonoid synthesis can be achieved by the treatment of B for Toona sinensis seedlings,effectively promoting the activities of PAL,CHS,and CHI enzymes.