非衍生-高效液相色谱法测定自来水中的草甘膦、双甘膦和增甘膦

Determination of glyphosate,N-phosphonomethyl aminodiacetic acid and glyphosine in tap water by non-derivatization high performance liquid chromatography

建立了一种非衍生-高效液相色谱法(HPLC)同时测定自来水中草甘膦、双甘膦和增甘膦的方法。自来水经微孔滤膜过滤后进样,采用聚合物基质的Shodex IC SI-50 4E(4.0×250 mm)强阴离子交换色谱柱分离,用碳酸氢铵溶液作为流动相进行洗脱,蒸发光散射检测器(ELSD)进行检测。草甘膦、双甘膦和增甘膦在0.4~40 mg·L^(-1)的范围内线性良好,相关系数均大于0.999。草甘膦、双甘膦和增甘膦的检出限分别为0.26 mg·L^(-1)、0.33 mg·L^(-1)和0.32 mg·L^(-1)。在1 mg·L^(-1)、2 mg·L^(-1)和10 mg·L^(-1)加标浓度下,草甘膦、双甘膦和增甘膦的平均回收率分别为103.2%~120.9%、83.6%~96.6%和83.7%~104.1%,相对标准偏差(RSD)分别为2.7%~5.1%、4.9%~8.2%和3.9%~7.0%。该方法可为自来水中草甘膦、双甘膦和增甘膦的测定提供参考。

A non-derivatization high performance liquid chromatography(HPLC)method for simultaneous determination of glypho-sate,n-phosphonomethyl aminodiacetic acid(PMIDA)and glyphosine in tap water was established.The tap water was treated by mi-crofiltration membrane filters and then sampled.Separation was carried out by a Shodex IC SI-504E(4.0x250 mm)strong anion ex-change column,which matrix is polymer.Ammonium bicarbonate solution was used as mobile phase for elution.The evaporative light scattering detector(ELSD)was used for detection.Glyphosate,PMIDA and glyphosine had good linearity in range of 0.4 to 40 mg:L^(-1),and the correlation coefficients were all greater than O.999.The detection limits of glyphosate,PMIDA and glyphosine were 0.26,0.33 and 0.32 mg·L^(-1),respectively.The average recoveries of glyphosate,PMIDA and glyphosine were 103.2%~120.9%,83.6%~96.6%and 83.7%~104.1%at 1 mg·L^(-1),2 mg·L^(-1)and 10 mg·L^(-1)spiked levels,respectively.And the relative standard deviations were(RSD)were 2.7%~5.1%,4.9%~8.2%and 3.9%~7.0%,respectively.This method can provide reference for the determination of glyphosate,PMIDA and glyphosine in tap water.