辽宁绒山羊产气荚膜梭菌的分离鉴定与耐药性分析

Isolation,identification and antibiotic resistance analysis of Clostridium perfringens from Liaoning cashmere goat

研究对疑似产气荚膜梭菌感染的辽宁绒山羊病例样本进行细菌分离鉴定,以期为辽南地区辽宁绒山羊养殖过程中科学防治产气荚膜梭菌病提供参考。试验采集腹泻辽宁绒山羊的粪便肛拭子和腹泻病死羊肠道内容物,结合临床症状进行染色镜检、PCR扩增、测序及药敏试验。结果显示,样本经细菌分离培养、染色镜检、鉴别培养、PCR鉴定确诊为产气荚膜梭菌感染。药敏试验结果显示,分离株对红霉素、四环素、诺氟沙星等耐药,对氟苯尼考、氯霉素和哌拉西林等敏感。研究表明,导致该辽宁绒山羊养殖场内羊腹泻及死亡的病原为产气荚膜梭菌,可根据药敏试验结果进行用药,避免出现耐药性影响治疗效果。

The bacterial isolation and identification of Liaoning cashmere goats suspected of Clostridium perfringens infection were studied in order to provide a reference for scientific prevention and control of Clostridium perfringens in the breeding process of Liaoning cashmere goats in southern Liaoning.The fecal anal swabs of diarrhea Liaoning cashmere goats and intestinal contents of diarrhea dead sheep were collected,and staining microscopic examination,PCR amplification,sequencing,and drug sensitivity test were carried out in combination with clinical symptoms.The results showed that the samples were confirmed as Clostridium perfringens infection by bacterial isolation and culture,staining and microscopic examination,differential culture and PCR identification.The results of drug sensitivity test showed that the isolates were resistant to erythromycin,tetracycline and norfloxacin,and sensitive to florfenicol,chloramphenicol and piperacillin.The study indicates that Clostridium perfringens is the pathogen causing diarrhea and death in Liaoning cashmere goat farm,which can be used according to the results of drug sensitivity test to avoid drug resistance affecting the treatment effect.