红曲治疗糖尿病网络药理学研究

Network Pharmacological Analysis of Monascus in the Treatment of Diabetes

目的:探讨红曲治疗糖尿病的内在机理。方法:通过搜索化源网、SwissTargetPrediction和GeneCards等数据库,应用Venny、STRING、Metascape、微生信、Cytoscape 3.7.2和AutoDock软件,开展网络药理学分析和分子对接实验研究。结果:通过筛选去重获得红曲活性成分与“diabetes”相关的潜在靶点488个,GO富集分析功能条目60条,其中涉及分子功能、生物过程和细胞组成的条目分别为20条、20条和20条。KEGG信号通路富集靶点数和显著性均较高的是癌症通路、神经活性配体-受体相互作用、细胞凋亡等通路。分子对接实验证明红曲主要活性成分可与SRC、STAT3和HSP90AA1靶点结合。结论:红曲主要活性成分可能通过SRC、STAT3和HSP90AA1等关键靶点治疗糖尿病,为进一步应用红曲治疗糖尿病提供了理论参考。

Objective:To explore the internal mechanism of Monascus in treating diabetes.Methods:The databases of Internet of Huayuan,SwissTargetPrediction and GeneCards,and the softwares of Venny,STRING,Metascape,Weishenxin,Cytoscape 3.7.2 and AutoDock were used to carry out the network pharmacological analysis and molecular docking.Results:The results showed that 488 potential targets related to “diabetes” and active components of Monascus were obtained through screening and elimination of duplication,and 60 functional items were identified by GO enrichment analysis.There were 20,20 and 20 items involving in molecular function,biological process and cell composition,respectively.The number of targets and enrichment significance of KEGG analysis were higher in cancer pathway,neural active ligand-receptor interaction,apoptosis and other pathways.Molecular docking experiments have proved that the main active ingredients of Monascus could bind to SRC,STAT3 and HSP90AA1 targets.Conclusion:Monascus may treat diabetes through key targets such as SRC,STAT3 and HSP90AA1.This provides theoretical reference for further study of Monascus in the treatment of diabetes.