低氧预适应对小鼠自然杀伤细胞活性的影响

Effect of hypoxic preconditioning on NK cell killing activity in mice

目的:探讨低氧预适应(hypoxic preconditioning,HPC)条件下对小鼠自然杀伤细胞(natural killer cell,NK)杀伤活性与功能的影响。方法:将小鼠分对照组(A组)、急性低氧组(B组)和HPC组(C组)3组,通过MTT比色法分别检测3组小鼠NK细胞的杀伤活性,通过EILSA检测NK细胞分泌干扰素-γ,Real-time PCR检测穿孔素、颗粒酶的表达量,并采用SPSS 19.0统计学软件进行数据分析。结果:低氧预适应组与急性低氧组相比NK杀伤力较强(P<0.05),其中12 h时NK细胞活性明显增强(P<0.05)。小鼠NK细胞分泌IFN-γ从4 h到16 h均逐渐下降(P<0.05),显示低氧预适应组随着时间的延长较低氧组更能适应低氧;HPC组与B组相比低氧损伤减轻,分泌穿孔素、颗粒酶表达多于低氧组(P<0.05)。结论:初步确定NK细胞在HPC与低氧组相比,NK细胞杀伤功能有所恢复;IFN-γ、穿孔素、颗粒酶蛋白的分泌量均有所升高。

Objective:To investigate the effect of hypoxic preconditioning(HPC)on NK cell killing activity in mice.Methods:Selected mice were divided into the control group(Group A),acute hypoxic group(Group B)and HPC group(Group C).The levels of interferon-γsecreted by NK cells were detected with EILSA,expression levels of perforin and granzyme were detected with Real-Time PCR,and the killing activities of NK cells of mice in the three groups were detected by MTT colorimetry.SPSS 19.0 statistical software was used for data analysis.Results:Compared with the group B,the killing activity of HPC group(Group C)was more powerful,and it obviously enhanced at 12 h(P<0.05).The secretion of IFN-γby NK cells was gradually decreased from 4 h to 16 h,which indicated that hypoxia preconditioning was more adaptive to hypoxia than the group B over time.Compared with the group B,the hypoxic injury of mice in the HPC group were relieved,and expression levels of perforin and granzyme were higher than that in the group B(P<0.05).Conclusion:Compared with the acute hypoxic group,NK cell killing function in the HPC group was recovered,and secretions of IFN-γ,perforin and granzyme protein were increased.