宫颈癌组织GOLPH3蛋白表达与铂类化疗敏感性的关系分析

Analysis of the relationship between GOLPH3 protein expression and platinum chemotherapy sensitivity in cervical cancer tissue

目的探究宫颈癌组织高尔基磷酸化蛋白3(GOLPH3)表达与铂类化疗敏感性的关系。方法选取2017年10月至2020年2月于青海红十字医院就诊的118例宫颈癌患者为研究对象。免疫组化法、Western blot检测GOLPH3表达情况;分析癌组织GOLPH3表达与病理特征的关系;比较不同化疗效果宫颈癌患者组织中GOLPH3阳性表达情况;Kaplan-Meier法探究GOLPH3表达与宫颈癌患者预后关系。结果宫颈癌组织中GOLPH3阳性表达率[70.34%(83/118)]、蛋白表达量(0.96±0.27)均高于癌旁组织[35.59%(42/118),0.58±0.16](P<0.05);癌组织GOLPH3表达与FIGO分期、分化程度、淋巴结转移、浸润深度相关;耐药组患者GOLPH3蛋白表达量显著高于敏感组患者(P<0.05);GOLPH3阳性表达患者3年生存率[72.29%(60/83)]低于阴性表达患者[91.43%(32/35)](P<0.05)。结论宫颈癌组织中GOLPH3阳性表达率及蛋白质均呈低表达水平,且其蛋白质表达量与铂类化疗敏感性密切相关,GOLPH3可能成为预测宫颈癌患者铂类化疗敏感性的分子标志物。

Objective To investigate the relationship between the expression of Golgi phosphorylated protein 3(GOLPH3)and platinum chemotherapy sensitivity in cervical cancer tissue.Methods A total of 118 patients with cervical cancer who visited Qinghai Red Cross Hospital from October 2017 to February 2020 were collected as the study objects.GOLPH3 expression was detected by immunohistochemistry and Western blot;to analyze the relationship between GOLPH3 expression and the pathological features in cancer tissues;the positive expression of GOLPH3 in tissues of cervical cancer patients with different chemotherapy effects was compared.Kaplan-Meier method to explore the relationship between the expression of GOLPH3 and prognosis of patients with cervical cancer.Results The positive expression of GOLPH3 in cervical cancer tissue[70.34%(83/118)]and protein expression(0.96±0.27)were higher than that in adjacent tissues[35.59%(42/118),0.58±0.16](P<0.05).GOLPH3 expression in cervical cancer tissue was correlated with FIGO stages,differentiation levels,lymph node metastasis,and depth of infiltration(P<0.05).The expression level of GOLPH3 protein in the drug resistant group was obviously higher than that in the sensitive group(P<0.05).The 3-year survival rate of patients with positive GOLPH3 expression[72.29%(60/83)]was lower than that of patients with negative expression[91.43%(32/35)](P<0.05).Conclusion GOLPH3-positive expression and protein were at low expression levels in cervical cancer tissues,and its protein expression level is closely related to platinum chemotherapy sensitivity.GOLPH3 may become a molecular marker for predicting platinum chemotherapy sensitivity in cervical cancer patients.