辽宁省700例HIV-1抗体确证阳性者免疫印迹试验条带分析

Analysis on western blot band patterns of 700 infected people with HIV-1 antibody positive in Liaoning province

目的分析辽宁省HIV-1抗体阳性样本免疫印迹试验(WB)带型分布特点,了解本省HIV-1感染者的带型分布规律及特征。方法收集2013—2020年在辽宁省疾病预防控制中心确证实验室进行WB检测HIV-1抗体阳性的感染者700例,对WB带型进行统计分析。结果700例HIV-1感染者中,出现9条带(全带型)比例最高,为36.9%;7条带及以上占总感染者数量的89.3%。env抗体检出率:gp160、gp120、gp41分别为100.0%、100.0%、99.7%,pol抗体检出率:p66、p51、p31分别为94.7%、91.1%、94.4%,gag抗体检出率:p24为98.7%,p55和p17检出率较低,分别为47.0%和66.0%。女性p55检出率高于男性,差异有统计学意义(P<0.05);不同年龄组感染者的p66、p51、p55、p24和p17的检出率差异均有统计学意义(P<0.05),其中p66、p51和p55在老年组检出率最高,p66、p51、p24和p17在未成年组检出率最高;不同人群的p55、p24和p17检出率差异有统计学意义(P<0.05)。结论辽宁省HIV-1抗体阳性样本中,7条带及以上带型较多,不同性别、年龄和不同人群感染者WB带型存在差异,中青年及男性人群为本地区的高危人群。

Objective To analyze band patterns of HIV-1 antibody positive samples tested by western blot(WB),to understand the distribution pattern and characteristics of HIV-1 infected patients in Liaoning province.Methods Statistical analysis was carried out for band patterns of HIV-1 antibody positive samples detected by WB in the confirmed laboratory of Liaoning Provincial Center for Disease Control and Prevention from 2013 to 2020.Results In 700 samples of HIV-1 antibody positive,36.9%were total bands,89.3%were more than 7 bands.The positive rate of anti-env:gp160 was 100.0%,gp120 was 100.0%,gp41 was 99.7%.The positive rate of anti-pol:p66 was 94.7%,p51 was 91.1%,p31 was 94.4%.Of the anti-gag,except p24 was 98.7%,p55 and p17 positive rate were low,p55 was 47.0%,p17 was 66.0%.The positive rate of p55 in female was higher than that in male,the difference was statistically significant(P<0.05).The difference in the positive rate of p66,p51,p55,p24 and p17 between different ages was statistically significant(P<0.05).The positive rate of p66,p51 and p55 were the highest in the eldly aged group,and p66,p51,p24 and p17 were the highest in the juvenile group.The differences of positive rate of p55,p24 and p17 appeared statistically significances in different populations(P<0.05).Conclusion Most of the HIV-1 antibody positive samples,possessed 7 bands or more.There was significant difference in the detection of WB bands patlerns between the people with different gender,age and population.Young and middle aged and male populations were at high risk of HIV-1infection.