基于代谢组学的白黄链霉菌TD-1高产帕马霉素代谢机制

Metabolic Mechanism of Streptomyces alboflavus T D-1 with High Pamamycin Production Based on Metabonomics

真菌毒素给食品安全带来了重大威胁,帕马霉素对病原真菌有良好的生物抑菌活性。为提高帕马霉素产量,基于代谢组学分析方法,研究了白黄链霉菌(Streptomyces alboflavus)T D-1在两种不同发酵培养基(添加与不添加1,2-丙二醇)中生长及代谢、胞内代谢产物及代谢通路的差异,对代谢产物的变化进行了评估和分析,结合显著差异代谢产物含量变化与代谢通路的交互关系,揭示了帕马霉素生物合成的高产机理。结果表明:培养基添加1,2-丙二醇后,白黄链霉菌T D-1产帕马霉素产量明显提高。利用气相色谱-质谱联用技术,鉴定出27种显著差异胞内代谢产物与帕马霉素生物合成密切相关,主要参与中心碳代谢、氨基酸代谢及脂肪酸代谢通路。此外,通过采用添加主要显著差异前体物策略,添加谷氨酸使帕马霉素产量提高102.33%。基于代谢组学分析确定添加前体物方法,可有效提高帕马霉素产量。本研究旨在为防治粮食加工食品的黄曲霉及黄曲霉毒素污染,从源头保障食品安全提供技术参考。

Mycotoxin present a significant risk to the safety of food,while pamamycin exhibits promising biological antibacterial properties against pathogenic fungi.In order to enhance the production of pamamycin,the differences in growth and metabolism,intracellular metabolites,and metabolic pathways of Streptomyces alboflavus T D-1 in two distinct fermentation media,one containing 1,2-propanediol and the other without it were studied based on metabolomics analysis method.The alterations in metabolite levels were examined in conjunction with the interplay between significantly different metabolite content changes and metabolic pathways to elucidate the underlying mechanism responsible for the high-yield biosynthesis of pamamycin.The findings of this study indicated that the addition of 1,2-propanediol increased the production of pamamycin by Streptomyces alboflavus T D-1.Through the utilization of gas chromatography-mass spectrometry,a total of 27 intracellular metabolites were identified as significantly different,and these metabolites were found to be closely associated with the biosynthesis of pamamycin.These metabolites were primarily involved in central carbon metabolism,amino acid metabolism,and fatty acid metabolism pathways.Furthermore,by employing a verification strategy of adding significant different precursors,the supplementation of glutamic acid resulted in a 102.33%increase in pamamycin yield.The supplementation of significantly different precursors,based on analysis of metabolomics,proved to be an effective approach for enhancing the yield of pamamycin.The objective of this study was to mitigate the adverse effects of Aspergillus flavus and aflatoxins contamination on grain processing foods,while also to ensure food safety from the source.