SKA2对宫颈癌细胞增殖、迁移和侵袭的影响及其预后价值

Impact of SKA2 on proliferation, migration and invasion of cervical cancer cells and its prognostic value

目的探究纺锤体和动粒相关复合物亚基2(SKA2)在宫颈癌组织中的表达和预后价值,及其对宫颈癌细胞增殖、迁移和侵袭的影响。方法通过生物信息学数据库及免疫组化SP法分析SKA2在宫颈癌组织中的表达,并分析其表达水平与宫颈癌患者临床病理特征的关系及其预后价值。RT-qPCR检测SKA2在人正常宫颈细胞(HcerEpic)以及宫颈癌细胞(HeLa、SiHa、CaSki、C-33A)中的mRNA表达,选取SKA2表达水平较高的宫颈癌细胞SiHa作为研究对象。构建下调SKA2表达的SiHa细胞模型,并验证其敲低效果,CCK-8法检测细胞增殖能力,细胞划痕实验检测细胞迁移能力,Transwell实验检测细胞迁移和侵袭能力。结果宫颈癌组织和细胞中SKA2的mRNA和蛋白表达水平较正常宫颈组织及细胞增高,差异有统计学意义(P<0.05)。SKA2高表达与宫颈癌患者的FIGO分期相关。敲低SKA2可抑制宫颈癌SiHa细胞的增殖、迁移和侵袭(P<0.05)。结论SKA2在宫颈癌组织和细胞中表达上调,并能够促进宫颈癌细胞增殖、迁移和侵袭。SKA2的表达水平与宫颈癌的进展相关,SKA2高表达的宫颈癌患者预后较差。

Objective To investigate the expression and prognostic value of spindle and kinetochore-associated complex subunit 2(SKA2)in cervical cancer tissues,as well as its impact on the proliferation,migration and invasion of cervical cancer cells.Methods The expression of SKA2 in cervical cancer tissues was analyzed by bioinformatics database and immunohistochemical SP method,and the relationship between SKA2 expression level and clinicopathological features of cervical cancer patients and its prognostic value was analyzed.The mRNA expression of SKA2 in human normal cervical cells(HcerEpic)and cervical cancer cells(HeLa,SiHa,CaSki,C-33A)was detected by RT-qPCR.Cervical cancer cells SiHa with higher SKA2 expression level was selected for further study.SiHa cell model with down-regulated SKA2 expression was constructed,and its knockdown effect was verified.Cell proliferation capacity was detected by CCK-8 method,cell migration capacity was detected by cell scratch wound healing assay,and cell migration and invasion capacity was detected by Transwell assay.Results Compared with normal cervical tissues and cells,the expression levels of SKA2 mRNA and protein were higher in cervical cancer tissues and cells,and the differences were statistically significant(P<0.05).High SKA2 expression was associated with FIGO staging in patients with cervical cancer.Furthermore,SKA2 knockdown could inhibit the proliferation,migration and invasion of SiHa cells in cervical cancer(P<0.05).Conclusion SKA2 is up-regulated in cervical cancer tissues and cells,and can promote the proliferation,migration and invasion of cervical cancer cells.The expression level of SKA2 is associated with the progression of cervical cancer,and the prognosis of cervical cancer patients with high SKA2 expression is worse.