磁压榨技术建立兔直肠阴道瘘模型

Establishment of rabbit rectovaginal fistula model by magnetic compression technique

目的探讨利用磁压榨技术建立兔直肠阴道瘘动物模型的可行性。方法自行设计适用于制备兔直肠阴道瘘模型的磁性装置。以8只新西兰雌兔作为实验对象,耳缘静脉注射麻醉后取仰卧位,分别经阴道及肛门将子、母磁体置于直肠阴道隔两侧,并使子、母磁体对位相吸。记录手术操作时间,观察术后实验兔一般状态,记录磁体排出时间,术后1周处死实验兔获取直肠阴道瘘标本,肉眼观察直肠阴道瘘形成情况。结果8只实验兔成功制备了直肠阴道瘘动物模型,手术操作过程顺利,平均用时(1.63±0.70)min,术后兔一般状况良好。术后(4.63±0.99)d磁体排出体外,术后1周获取直肠阴道瘘标本,肉眼观察可见直肠阴道瘘形成良好。结论利用磁压榨技术建立兔直肠阴道瘘模型具有操作简单、模型制备成功率高的优点。

Objective To investigate the feasibility of establishing an animal model of rectovaginal fistula in rabbits by magnetic compression technique.Methods A magnetic device suitable for preparing rabbit rectovaginal fistula model was self-designed.Eight New Zealand female rabbits were used as experimental subjects.They were placed in a supine position after auricular intravenous anesthesia,and the magnets were placed on both sides of the rectovaginal septum through the vagina and anus,respectively,and the magnets were made to attract together.The operation time was recorded,the general state of the experimental rabbits was observed after operation,and the time of magnet discharge was recorded.The experimental rabbits were killed 1 week after operation to obtain rectovaginal fistula specimens,and the formation of rectovaginal fistula was observed by naked eye.Results The animal model of rectovaginal fistula was successfully established in 8 experimental rabbits.The operation process was smooth,with an average time of(1.63±0.70)minutes.The rabbits were generally in good condition after operation.The magnet was discharged from the body at(4.63±0.99)day after operation,and the rectovaginal fistula specimens were obtained 1 week after operation,and the rectovaginal fistula was well formed by naked eye observation.Conclusion The establishment of rabbit rectovaginal fistula model by magnetic compression technique has the advantages of simple operation and high success rate of model preparation.