豇豆品种在盐胁迫下叶片叶绿素荧光特性聚类分析

Cluster Analysis of Chlorophyll Fluorescence Characteristics of Vigna unguiculata Varieties under Salt Stress

以50个豇豆品种为试验材料,设置ck、NaCl、EBR与EBR+NaCl组等4个处理,于7 d后测量叶片叶绿素荧光参数,研究各处理下豇豆幼苗的叶片荧光特性。主成分分析表明,Y(Ⅱ)、Fv/Fm和Y(NO)可以代表豇豆的13个荧光参数。聚类分析结果显示,50个豇豆品种聚为Ⅰ类、Ⅱ类、Ⅲ类,Ⅰ类有46个品种,Ⅱ类有2个品种,Ⅲ类有2个品种;其中Ⅱ类的668绿条和豇豆WJ-60的Y(Ⅱ)与Fv/Fm值最高,Y(NO)值最低,表明光合作用效率高,光损伤程度低,是较为理想的豇豆品种;而Ⅲ类的青条和花荣的Y(Ⅱ)与Fv/Fm值最低,Y(NO)值最高,表明光合作用效率低,光损伤程度高。同时EBR对豇豆叶绿素荧光特性有影响,因品种不同而存在差异。

The chlorophyll fluorescence parameters of 50 Vigna unguiculata seedlings were measured after 7 days with ck, NaCl, EBR and EBR+NaCl treatments. The leaf fluorescence characteristics of Vigna unguiculata seedlings under each treatment were studied. Principal component analysis showed that Y(Ⅱ), F_(v)/F_(m) and Y(NO) could represent 13 fluorescence parameters of Vigna unguiculata. The results of cluster analysis showed that 50 Vigna unguiculata varieties were grouped into classⅠ, Class Ⅱ and Class Ⅲ. There were 46 varieties in class Ⅰ, 2 varieties in class Ⅱ and 2 varieties in class Ⅲ. Among them, the Y(Ⅱ) and F_(v)/F_(m) values of 668 green stripe and WJ-60 were the highest and the Y(NO) values were the lowest, indicating that the photosynthesis efficiency was high and the light damage degree was low, so they were ideal varieties. The Y(Ⅱ) and F_(v)/F_(m) values were the lowest and Y(NO) values were the highest, indicating low photosynthetic efficiency and high degree of light damage. At the same time, EBR had an effect on chlorophyll fluorescence characteristics of, which was different with different varieties.