蒲公英金纳米颗粒的制备及体外抗癌活性研究

Preparation and in vitro anticancer activity of dandelion gold nanoparticles

以蒲公英提取液为还原剂、氯金酸为原料合成了蒲公英金纳米颗粒(Da-AuNPs),采用多种手段对其进行了表征,结果表明:Da-AuNPs呈球形或近球形,平均粒径约为(21.2±3.0) nm;蒲公英中含丰富羟基的活性物质可能是合成Da-AuNPs的主要还原剂;金纳米颗粒具有典型的面心立方(FCC)晶格结构;Da-AuNPs悬液放置5 d,其粒径没有显著变化,体系具有较好的稳定性。采用噻唑蓝溴化四唑(MTT)试验对Da-AuNPs进行了体外细胞毒性测试,结果显示:在6.25~200μg/mL的范围内,Da-AuNPs对RAW264.7正常细胞具有良好的生物相容性;当Da-AuNPs的质量浓度为200μg/mL时,在808 nm的近红外线(1.5 W/cm^(2))下照射3 min,与对照组相比4T1癌细胞活力下降至65%,表明Da-AuNPs具有一定的体外抗癌活性。

Dandelion gold nanoparticles(Da-AuNPs) were synthesized by using dandelion extract as a reducing agent and chloroauric acid as raw material and characterized by various methods. The results show that Da-AuNPs are spherical or nearly spherical, and the average particle size is(21.2±3.0) nm. The active substances containing abundant hydroxyl groups in dandelions may be the main reducing agents for the synthesis of Da-AuNPs. Gold nanoparticles have a typical face-centered cubic(FCC) lattice structure. When a Da-AuNPs suspension was left for 5 days, its particle size did not change significantly, indicating that the system had good stability. The in vitro cytotoxicity of Da-AuNPs was tested by MTT assay. The results showed that Da-AuNPs had good biocompatibility to RAW264.7 normal cells in the range 6.25-200 μg/mL. After exposure to 808 nm near-infrared radiation(1.5 W/cm^(2)) for 3 min, when the mass concentration of Da-AuNPs was 200 μg/mL, the viability of 4T1 cancer cells decreased to 65% compared with the control group, indicating that Da-AuNPs had a certain anti-cancer activity in vitro.