橘皮对黄瓜苗期枯萎病的防效

Control Effects of Orange Peel on Cucumber Seedlings Fusarium Wilt

采用体外抑菌试验与盆钵培养试验相结合的方法,探究了橘皮与柚皮浸提液对尖孢镰刀菌生长及橘皮对黄瓜苗期枯萎病的防效,结果表明,橘皮浸提液对尖孢镰刀菌黄瓜专化型病原菌表现出较显著的抑制作用,且抑制作用与提取液浓度成正比。在PDA上接种病原菌后的第2天与第6天,对照的菌落直径分别为全浓度橘皮浸提液处理的1.58与1.54倍。橘皮的总黄酮含量是柚皮的1.14倍;此外,土壤强还原灭菌时添加0.25%橘皮可有效降低黄瓜苗期枯萎病,接种病原菌条件下,添加橘皮的幼苗地上部生物量较对照增加了15.49%,根系可溶性糖含量是对照的1.12倍。表明利用橘皮防治黄瓜苗期枯萎病有极大的应用潜力。

A combination of in vitro antibacterial tests and pot-culture experiments was conducted to explore the effects of orange and grapefruit peel extract on the growth of Fusarium oxysporum and potentiality of orange peel extract in preventing cucumber seedlings damping-off disease.The results showed that the orange peel extract exhibited a significant inhibitory effect on the Fusarium oxysporum cucumber-specializing pathogen,and the inhibitory effect was positively correlated with the concentration of the extract.On the 2nd and 6th day after pathogenic inoculating on PDA,the colony diameters of the control were 1.58 and 1.54 times larger than those of the full concentration of orange peel extract treatment.The total flavonoid content in orange peel was 1.14 times higher than that of grapefruit peel.Additionally,adding 0.25%orange peel to reduction sterilization soil could effectively reduce cucumber seedling wilt.Under the condition of pathogen infection,the total biomass of seedlings grown on reduction sterilization soil added orange peel increased by 15.49%compared with the control,and soluble sugar content in root was 1.12 times higher than that of the control.It was suggested that the application of orange peel to control cucumber seedling wilt has great potential.