核糖体蛋白L34和STAT3表达与宫颈癌临床病理特征及预后的关系

Expressions of ribosomal protein L34 and STAT3 in cervical cancer tissue and their relationships with clinicopathologic features and prognosis

目的探讨核糖体蛋白L34(RPL34)和信号转导和转录激活因子3(STAT3)表达与宫颈癌临床病理特征及预后的关系。方法回顾性收集2019年1月至2022年6月台州市中心医院(台州学院附属医院)手术切除的30例宫颈癌和30例正常宫颈(对照)新鲜组织,以及2017年1月至2022年6月手术切除的76例宫颈癌和40例正常宫颈(对照)组织石蜡标本。采用qRT-PCR法检测新鲜组织中RPL34和STAT3 mRNA相对表达量,采用免疫组化法检测石蜡标本中RPL34和STAT3蛋白表达水平,分析RPL34和STAT3在不同临床病理特征宫颈癌患者中的表达差异,采用Spearman秩相关分析RPL34和STAT3表达的相关性,绘制Kaplan-Meier生存曲线分析RPL34和STAT3表达的预后意义。结果宫颈癌组织中RPL34 mRNA相对表达量和蛋白表达水平均显著低于对照组织(均P<0.01),STAT3 mRNA相对表达量和蛋白表达水平均显著高于对照组织(均P<0.01)。不同国际妇产科学联盟(FIGO)分期、浸润深度及淋巴结转移患者宫颈癌组织中RPL34表达水平比较,差异均有统计学意义(均P<0.01),不同FIGO分期、浸润深度、淋巴结转移及分化程度患者宫颈癌组织中STAT3表达水平比较,差异均有统计学意义(均P<0.01)。宫颈癌组织中RPL34表达和STAT3表达呈显著负相关(P<0.05)。与阴性组患者相比,RPL34阳性组患者术后3年总生存率显著升高(P<0.05),STAT3阳性组患者术后3年总生存率显著降低(P<0.05)。结论宫颈癌组织中RPL34 mRNA和蛋白呈低表达,STAT3 mRNA和蛋白呈高表达,RPL34和STAT3表达与宫颈癌的浸润深度、淋巴结转移等临床病理特征有关,并影响患者3年总生存率。RPL34和STAT3有望成为宫颈癌预后判断的标志物。

Objective To investigate the expressions of ribosomal protein L34(RPL34)and signal transduction and transcription regulator 3(STAT3)in cervical cancer tissue and their relationships with clinicopathologic features and prognosis.Methods Cervical cancer tissues and normal cervical tissues(as control)were collected from Taizhou Central Hospital from January 2019 to June 2022,each from 30 patients;Paraffin specimens of 76 cases of cervical cancer and 40 normal cervical tissues(as control)were also collected from January 2017 to June 2022.The relative expressions of RPL34 and STAT3 mRNA in fresh tissue were detected by qRT-PCR,while the expressions of RPL34 and STAT3 in paraffin specimens were detected by immunohistochemistry so as to analyze the expression differences of RPL34 and STAT3 among cervical cancer patients with different clinicopathological characteristics.The correlation between RPL34 and STAT3 expressions was analyzed using Spearman rank correlation analysis,and Kaplan-Meier survival curves were drawn to analyze the prognostic significance of RPL34 and STAT3 expressions.Results The RPL34 mRNA relative expression and protein expression in cervical cancer tissue were significantly lower than those in normal control tissue(both P<0.01),while the STAT3 mRNA relative expression and protein expression were significantly higher than those in normal control tissue(both P<0.01).There were statistically significant differences in the expression level of RPL34 in cervical cancer patients with different International Federation of Gynecology and Obstetrics(FIGO)stages,depth of invasion and lymph node metastasis(all P<0.01),and statistically significant differences in the expression level of STAT3 in cervical cancer patients with different FIGO stages,depth of invasion,lymph node metastasis and differentiation(all P<0.01).The expression of RPL34 in cervical cancer tissue was negatively related to the expression of STAT3(P<0.01).Compared with patients with negative expression,those with positive RPL34 expression had a significantly higher 3-year overall survival rate after surgery(P<0.01),while those with positive STAT3 expression showed a significantly lower 3-year overall survival rate after surgery(P<0.01).Conclusion RPL34 mRNA and protein in cervical cancer tissue are down-regulated,while STAT3 mRNA and protein are up-regulated.The expressions of RPL34 and STAT3 are related to the clinicopathological characteristics including the depth of infiltration and lymph node metastasis of cervical cancer and so on,and they affect the 3-year overall survival rate of patients.RPL34 and STAT3 are expected to become prognostic markers for cervical cancer.