唾液乳杆菌Li01对苯并芘诱导长爪沙鼠胃炎的改善作用研究

Study on the improving effect of Ligilactobacillus salivarius Li01 on gastritis induced by benzo(a)pyrene in Mongolian gerbils

目的 建立苯并芘[benzo(a)pyrene, BaP]长时间诱导的长爪沙鼠胃炎模型,并探究唾液乳杆菌Li01对该模型的影响。方法 SPF级健康长爪沙鼠45只起初分为2组:正常对照组(NC,18只)和模型组(BaP, 27只),通过BaP灌胃造模32周后,从模型组中随机分出9只作为第3组(BaP-Li01组),用唾液乳杆菌Li01干预4周,期间记录不同组别长爪沙鼠毛发,体重等生长指标,第40周利用外周血彗星实验、流式细胞仪检测和组织病理切片探究BaP和唾液乳杆菌Li01对长爪沙鼠机体的影响。结果 经BaP灌胃后, BaP组长爪沙鼠毛发凌乱无光泽,出现耸毛、脱毛现象,而正常对照组长爪沙鼠毛发浓密光滑;另相比较于模型组,经唾液乳杆菌Li01干预后的BaP-Li01组长爪沙鼠毛发状况有所好转,接近正常对照组。与正常对照组相比,BaP组长爪沙鼠体重增长幅度明显变小, 16周后甚至小幅下降,经唾液乳杆菌Li01干预后, BaP-Li01组长爪沙鼠体重逐渐恢复。彗星实验和流式细胞凋亡检测发现, BaP摄入会造成长爪沙鼠细胞DNA损伤,而唾液乳杆菌Li01干预后可以改善、修复其损伤。病理学组织切片检查结果表明,BaP可诱发长爪沙鼠慢性胃炎,而唾液乳杆菌Li01的干预有助于胃炎的改善和修复。结论 BaP灌胃可成功建立长爪沙鼠的胃炎模型,而唾液乳杆菌Li01对其损伤有一定改善和修复作用。本研究为应用唾液乳杆菌Li01改善和修复胃炎提供了新思路。

Objective To establish a long-term benzo(a)pyrene(BaP)-induced gastritis model in Mongolian gerbils and investigate the effects of Ligilactobacillus salivarius Li01 on this model.Methods A total of 45 SPF healthy Mongolian gerbils were initially divided into 2 groups:The normal control group(NC,n=18),and the model group(BaP,n=27).After 32 weeks of BaP gavage to induce gastritis,9 gerbils were randomly selected from the model group to form a third group(BaP-Li01 group),which was orally administered Li01 for 4 weeks.During the experiment,growth indicators such as fur condition and body weight of Mongolian gerbils in different groups were recorded.In the 40th week,the peripheral blood comet assay,flow cytometry analysis,and histopathological examination were conducted to investigate the effects of BaP and Li01 on Mongolian gerbils.Results After BaP gavage,gerbils in the BaP group exhibited disheveled and dull fur with signs of piloerection and hair loss,whereas gerbils in the normal control group had dense and smooth fur.Compared to the model group,the BaP-Li01 group treated with Ligilactobacillus salivarius Li01 showed an improvement in the fur condition,approaching that of the normal control group.The weight gain of gerbils in the BaP group was significantly reduced compared to the normal control group,with even a slight weight loss after 16 weeks.After treatment with Ligilactobacillus salivarius Li01,the body weight of Mongolian gerbils in the BaP-Li01 group gradually recovered.Comet assay and flow cytometry apoptosis detection revealed that BaP gavage caused DNA damage in Mongolian gerbil cells,which was ameliorated and repaired following Ligilactobacillus salivarius Li01 intervention.Histopathological examination results indicated that BaP could induce chronic gastritis in Mongolian gerbils,while intervention with Li01 contributed to the improvement and repair of gastritis.Conclusion BaP gavage can successfully establish a gastritis model in Mongolian gerbils,while Ligilactobacillus salivarius Li01 demonstrates a protective and reparative effect on BaP-induced damage to a certain extent.This study provides a new perspective for the application of Ligilactobacillus salivarius Li01 in improving and repairing gastritis.