芜湖市腹泻患者沙门菌PFGE分子分型及耐药特征研究

PFGE molecular typing and drug resistance analysis of Salmonella from patients with diarrhea in Wuhu City

目的了解芜湖市腹泻患者沙门菌血清型、分子分型分布特征及耐药情况,为沙门菌监测提供依据。方法对芜湖市腹泻患者粪便中分离的40株沙门菌进行血清分型和脉冲场凝胶电泳(PFGE)分子分型分析并运用Bio Numeries 6.6软件进行聚类分析,采用革兰氏阴性菌药敏检测板进行细菌耐药性检测。结果40株沙门菌有7个血清群、11种血清型,最多的是D群肠炎沙门菌(14株),其次是B群鼠伤寒沙门菌(12株)和C1群蒙得维的亚沙门菌(5株)。经PFGE分型40株沙门菌共分成30个带型,带型同源性45.80%~100.00%,14株肠炎沙门菌带型有6种,12株鼠伤寒沙门菌带型有12种,血清型相同的沙门菌PFGE带型有同源性。肠炎沙门菌、里森沙门菌有成簇现象。40株沙门菌对萘啶酸耐药率最高,为60.00%,其次为氨苄西林、链霉素、四环素,多重耐药率达62.50%。结论芜湖市腹泻患者分离的沙门菌多种血清型和基因型同时流行,存在潜在暴发风险,耐药形势严峻,需加强对抗生素使用的监管力度,减少耐药株的出现和传播。

Objective To understand the serotype,molecular type distribution and drug resistance of Salmonella from patients with diarrhea in Wuhu City,and to provide the basis for Salmonella surveillance.Methods Serotyping and molecular typing analysis by pulsed-field gel electrophoresis(PFGE)were performed on 40 strains of Salmonella isolated from feces of diarrhea patients in Wuhu City during.The PFGE typing results were cluster analyzed by BioNumeries 6.6 software.The bacteria′s antimicrobial resistance was detected using a gram-negative drug susceptibility test plate.Results There were 7 serogroups and 11 serotypes in the 40 isolates,the most of which were 14 strains of Salmonella enteritidis in group D,12 strains of Salmonella typhimurium in group B and 5 strains of Salmonella Montevideo in group C1.The 40 strains of Salmonella were classified into 30 band types by PFGE,and the band type homology was 45.80%-100%.There were 6 band types of 14 strains of Salmonella enteritidis and 12 band types of 12 strains of Salmonella typhimurium.The PFGE band types of Salmonella with the same serotype were homologous.There were clusters of Salmonella enteritidis and Salmonella rishonensis.The drug resistance rate of 40 Salmonella strains to nalidixic acid was 60.00%,followed by ampicillin,streptomycin,tetracycline,and the multiple drug resistance rate reached 62.50%.Conclusion Multiple serotypes and genotypes of Salmonella isolated from diarrhea patients in Wuhu City are prevalent simultaneously,with potential outbreak risk and serious drug resistance situation.Antibiotic use management and supervision should be strengthened to reduce the emergence and spread of drug-resistant strains.