苹果病毒检测技术

Detection Methods of Apple Tree Viruses

简述了苹果病毒检测的指示植物检测法、电子显微镜检测技术、血清学检测技术、核酸杂交检测技术、PCR检测技术、逆转录重组酶聚合酶扩增检测技术、逆转录环介导恒温扩增检测技术、基因芯片检测技术、高通量测序检测技术的原理和特点,综述了不同检测技术取得的进展,展望了今后研究的主要方向。

In this paper,the principle and characteristics of detection techniques for apple tree virus were briefly summarized,including indicator plant method,transmission electron microscopy,serological detection,nucleic acid hybridization technique,reverse transcriptase polymerase amplification technique,reverse transcription recombinase polymerase amplification technique,reverse transcription-loop mediated thermostatic amplification technique,gene microarray and high-throughput sequencing technique.The progress of different technologies for apple tree viruses detection were reviewed.In addition,future research directions are prospected.