内源激素对扁蓿豆荚果开裂影响的研究

Effects of Endogenous Hormones on Pod Cracking of Medicago ruthenica

植物内源激素参与荚果的成熟与衰老过程。为探讨激素对扁蓿豆荚果裂荚性的影响,本试验以抗裂荚和易裂荚两种裂荚特性的科尔沁沙地扁蓿豆为材料,比较不同时期两种裂荚特性材料的荚果腹缝线处激素的差异,结果表明:扁蓿豆荚果腹缝线处共检测到生长素、乙烯前体(1-氨基环丙烷羧酸)、赤霉素、脱落酸、细胞分裂素、水杨酸和茉莉酸等7类植物激素;在整个荚果发育时期,抗裂荚材料荚果腹缝线的生长素总含量、乙烯前体(1-氨基环丙烷羧酸)含量和赤霉素含量均高于易裂荚材料;脱落酸含量在第20 d出现了抗裂荚材料高于易裂荚材料的现象,细胞分裂素、水杨酸和茉莉酸含量的变化则相反;主成分分析表明,前3个主成分的贡献率累计达78.15%,特征值总和为5.471,基本概括了7类激素的主要信息。

Plant endogenous hormones are involved in the maturation and senescence of pods.In order to explore the effect of hormones on pod cracking of Medicago ruthenica,this experiment used two kinds of pod cracking characteristics of Medicago ruthenica Trautv cv.Keerqinshadi(anti cracking and easy crack⁃ing)as materials to compare the difference of hormones at the ventral suture of the two pods with different pod cracking characteristics at different stages.The results showed that seven major types of phytohor⁃mones,including auxin,ethylene precursor(1-aminocyclopropane carboxylic),gibberellin,abscisic acid,cytokinin,salicylic acid and jasmonic acid were detected at the ventral suture of M.ruthenica pods;During the whole pod development period,the total content of auxin,the content of ethylene precursor 1-aminocy⁃clopropanecarboxylic acid,and the content of gibberellin at ventral suture of anti cracking pod were higher than those in easy cracking pod materials.The content of abscisic acid on the 20th day showed a phenom⁃enon where the anti cracking pod material was higher than the easy cracking pod material,while the changes in the content of cytokinin,salicylic acid and jasmonic acid were opposite.Principal component analysis showed that the cumulative contribution rate of the first three principal components was 78.15%,with a total eigenvalue of 5.471,which basically summarized the main information of the seven hormones measured.